Abstract
We synthesized highly stable, amphiphilic polymer-protected DNA-Au nanoparticle conjugates (DNA-AuNPs) and developed a DNA encoding/decoding strategy using these particles. Here, DNA-AuNPs were encapsulated by amphiphilic poly(N-vinyl-2-pyrrolidone) (PVP) to make them more stable and soluble in both organic and aqueous solvents. These particles are stable in a wide range of salt concentrations, pH and temperatures and sustain their stability even after the addition of d,l-dithiothreitol. Finally, this polymer layer was readily removed and particle-modified DNA was decoded via the microarrayed chip and dark-field-based AuNP imaging technique.
Original language | English |
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Pages (from-to) | 9467-9470 |
Number of pages | 4 |
Journal | Journal of Materials Chemistry |
Volume | 21 |
Issue number | 26 |
DOIs | |
Publication status | Published - 2011 Jul 14 |
Externally published | Yes |
ASJC Scopus subject areas
- General Chemistry
- Materials Chemistry