Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap

Dong Kwon Lim; Ki Seok Jeon; Jae Ho Hwang; Hyoki Kim; Sunghoon Kwon; Yung Doug Suh; Jwa Min Nam

doi:10.1038/nnano.2011.79

Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap

Dong Kwon Lim, Ki Seok Jeon, Jae Ho Hwang, Hyoki Kim, Sunghoon Kwon, Yung Doug Suh, Jwa Min Nam

Research output: Contribution to journal › Article › peer-review

968 Citations (Scopus)

Abstract

An ideal surface-enhanced Raman scattering (SERS) nanostructure for sensing and imaging applications should induce a high signal enhancement, generate a reproducible and uniform response, and should be easy to synthesize. Many SERS-active nanostructures have been investigated, but they suffer from poor reproducibility of the SERS-active sites, and the wide distribution of their enhancement factor values results in an unquantifiable SERS signal. Here, we show that DNA on gold nanoparticles facilitates the formation of well-defined gold nanobridged nanogap particles (Au-NNP) that generate a highly stable and reproducible SERS signal. The uniform and hollow gap (∼1 nm) between the gold core and gold shell can be precisely loaded with a quantifiable amount of Raman dyes. SERS signals generated by Au-NNPs showed a linear dependence on probe concentration (R²>0.98) and were sensitive down to 10 fM concentrations. Single-particle nano-Raman mapping analysis revealed that >90% of Au-NNPs had enhancement factors greater than 1.0 × 10 ⁸, which is sufficient for single-molecule detection, and the values were narrowly distributed between 1.0 × 10⁸ and 5.0 × 10⁸.

Original language	English
Pages (from-to)	452-460
Number of pages	9
Journal	Nature Nanotechnology
Volume	6
Issue number	7
DOIs	https://doi.org/10.1038/nnano.2011.79
Publication status	Published - 2011 Jul
Externally published	Yes

Bibliographical note

Funding Information:
D-K.L. acknowledges financial support from the CJ Pharmaceutical Research Institute. K-S.J. acknowledges support by the Public Welfare & Safety Research Program through NRF funded by MEST (2010-0020-795).

Funding Information:
Y.D.S. was supported by KRICT (KK-0904-02, SI-1110), the Nano R&D Program (No. 2009-0082861), the Pioneer Research Center Program of NRF (No. 2009-0081511), the Development of Advanced Scientific Analysis Instrumentation Project of KRISS by MEST and the Eco-technopia 21 Project by KME. J-M.N. was supported by the 21C Frontier Functional Proteomics Project (FPR08-A2-150) and the Nano R&D program (2008-02890) through the National Research Foundation of Korea (NRF) from the Ministry of Education, Science and Technology. The authors would also like to acknowledge financial support from the Industrial Core Technology Development Program of the Ministry of Knowledge Economy (nos 10033183 and 10037397) and the KRICT OASIS Project from the Korea Research Institute of Chemical Technology.

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Materials Science(all)
Condensed Matter Physics
Electrical and Electronic Engineering

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10.1038/nnano.2011.79

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@article{75bec81ee4ca466d88360adb0737c6ef,

title = "Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap",

abstract = "An ideal surface-enhanced Raman scattering (SERS) nanostructure for sensing and imaging applications should induce a high signal enhancement, generate a reproducible and uniform response, and should be easy to synthesize. Many SERS-active nanostructures have been investigated, but they suffer from poor reproducibility of the SERS-active sites, and the wide distribution of their enhancement factor values results in an unquantifiable SERS signal. Here, we show that DNA on gold nanoparticles facilitates the formation of well-defined gold nanobridged nanogap particles (Au-NNP) that generate a highly stable and reproducible SERS signal. The uniform and hollow gap (∼1 nm) between the gold core and gold shell can be precisely loaded with a quantifiable amount of Raman dyes. SERS signals generated by Au-NNPs showed a linear dependence on probe concentration (R2>0.98) and were sensitive down to 10 fM concentrations. Single-particle nano-Raman mapping analysis revealed that >90% of Au-NNPs had enhancement factors greater than 1.0 × 10 8, which is sufficient for single-molecule detection, and the values were narrowly distributed between 1.0 × 108 and 5.0 × 108.",

author = "Lim, {Dong Kwon} and Jeon, {Ki Seok} and Hwang, {Jae Ho} and Hyoki Kim and Sunghoon Kwon and Suh, {Yung Doug} and Nam, {Jwa Min}",

note = "Funding Information: D-K.L. acknowledges financial support from the CJ Pharmaceutical Research Institute. K-S.J. acknowledges support by the Public Welfare & Safety Research Program through NRF funded by MEST (2010-0020-795). Funding Information: Y.D.S. was supported by KRICT (KK-0904-02, SI-1110), the Nano R&D Program (No. 2009-0082861), the Pioneer Research Center Program of NRF (No. 2009-0081511), the Development of Advanced Scientific Analysis Instrumentation Project of KRISS by MEST and the Eco-technopia 21 Project by KME. J-M.N. was supported by the 21C Frontier Functional Proteomics Project (FPR08-A2-150) and the Nano R&D program (2008-02890) through the National Research Foundation of Korea (NRF) from the Ministry of Education, Science and Technology. The authors would also like to acknowledge financial support from the Industrial Core Technology Development Program of the Ministry of Knowledge Economy (nos 10033183 and 10037397) and the KRICT OASIS Project from the Korea Research Institute of Chemical Technology.",

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AU - Jeon, Ki Seok

AU - Hwang, Jae Ho

AU - Kim, Hyoki

AU - Kwon, Sunghoon

AU - Suh, Yung Doug

AU - Nam, Jwa Min

N1 - Funding Information: D-K.L. acknowledges financial support from the CJ Pharmaceutical Research Institute. K-S.J. acknowledges support by the Public Welfare & Safety Research Program through NRF funded by MEST (2010-0020-795). Funding Information: Y.D.S. was supported by KRICT (KK-0904-02, SI-1110), the Nano R&D Program (No. 2009-0082861), the Pioneer Research Center Program of NRF (No. 2009-0081511), the Development of Advanced Scientific Analysis Instrumentation Project of KRISS by MEST and the Eco-technopia 21 Project by KME. J-M.N. was supported by the 21C Frontier Functional Proteomics Project (FPR08-A2-150) and the Nano R&D program (2008-02890) through the National Research Foundation of Korea (NRF) from the Ministry of Education, Science and Technology. The authors would also like to acknowledge financial support from the Industrial Core Technology Development Program of the Ministry of Knowledge Economy (nos 10033183 and 10037397) and the KRICT OASIS Project from the Korea Research Institute of Chemical Technology.

PY - 2011/7

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N2 - An ideal surface-enhanced Raman scattering (SERS) nanostructure for sensing and imaging applications should induce a high signal enhancement, generate a reproducible and uniform response, and should be easy to synthesize. Many SERS-active nanostructures have been investigated, but they suffer from poor reproducibility of the SERS-active sites, and the wide distribution of their enhancement factor values results in an unquantifiable SERS signal. Here, we show that DNA on gold nanoparticles facilitates the formation of well-defined gold nanobridged nanogap particles (Au-NNP) that generate a highly stable and reproducible SERS signal. The uniform and hollow gap (∼1 nm) between the gold core and gold shell can be precisely loaded with a quantifiable amount of Raman dyes. SERS signals generated by Au-NNPs showed a linear dependence on probe concentration (R2>0.98) and were sensitive down to 10 fM concentrations. Single-particle nano-Raman mapping analysis revealed that >90% of Au-NNPs had enhancement factors greater than 1.0 × 10 8, which is sufficient for single-molecule detection, and the values were narrowly distributed between 1.0 × 108 and 5.0 × 108.

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Highly uniform and reproducible surface-enhanced Raman scattering from DNA-tailorable nanoparticles with 1-nm interior gap

Abstract

Bibliographical note

ASJC Scopus subject areas

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