Histamine release and inflammatory cell infiltration in airway mucosa in methylene diphenyl diisocyanate (MDI)-induced occupational asthma

Gyu Young Hur, Seung Soo Sheen, Young Mi Kang, Dong Hee Koh, Han Jung Park, Young Min Ye, Hyun Ee Yim, Kyoo Sang Kim, Hae Sim Park

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Introduction: Although methylene diphenyl diisocyanate (MDI) is widely used in industries, there have been few studies of the pathogenic mechanisms of MDI-induced occupational asthma (MDI-OA). Methods: We performed immunohistochemical analyses, measured inflammatory mediators and cytokines, and quantified histamine release (HR) from peripheral basophils in MDI-OA patients. Thirteen MDI-exposed workers (five MDI-OA, two MDI-induced esoinophilic bronchitis, and six asymptomatic exposed controls, AEC) were enrolled. Results and Discussion: Immunochemical analyses indicated significantly increased anti-eosinophilic cationic protein-stained cells in MDI-OA patients as compared with controls (P<0.05). Sputum eosinophil cationic protein levels were increased after MDI-specific inhalation challenge test in MDI-OA/EB patients (P<0.02). Sputum eosinophil counts were highly correlated with IL-8 and MMP-9 levels (P<0.05 and P<0.01, respectively). Basophil HR was significantly increased in MDI-OA patients after stimulations with anti-IgG4 and MDI-human serum albumin conjugates (both P<0.05). Eosinophil activation is a major feature of airway inflammation in MDI-OA patients. Increased HR by MDI may contribute to the pathogenic mechanisms of MDI-OA.

Original languageEnglish
Pages (from-to)571-580
Number of pages10
JournalJournal of Clinical Immunology
Issue number5
Publication statusPublished - 2008 Sept
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgment We would like to thank Dr. Adam Wisnewski of Yale University for providing the MDI–HSA conjugates used in this study. This study was supported by a grant from the Korean Health 21 R&D Project of the Ministry of Health and Welfare, Republic of Korea (A050571).


  • Eosinophil
  • MDI
  • Mast cell
  • Occupational asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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