Homogenous growth of gold nanocrystals for quantification of PSA protein biomarker

Cuong Cao, Xinxing Li, Jeewon Lee, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


We report on another alternative sensing platform for the detection of protein biomarker (PSA-ACT complex) based on homogenous growth of Au nanocrystals in solution phase. The immuno-recognition event is translated into the gold nanoparticle growth signal which can be intuitively recognized by an unaided eye, or quantitatively measured by an UV-vis spectrophotometric analysis. Surface plasmonic signature and kinetics of the Au nanogrowth in the homogenous phase containing of HAuCl4, AA, and CTAB have also been studied to provide suitable parameters for the immunoassay. As a result, detection limit of PSA-ACT complex was determined to be 10 fM. The result indicated that this is a very sensitive, robust, simple, and economic strategy to detect protein biomarkers, and it has great potential to detect other biological interactions.

Original languageEnglish
Pages (from-to)1292-1297
Number of pages6
JournalBiosensors and Bioelectronics
Issue number5
Publication statusPublished - 2009 Jan 1

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0/ROA-2007-000-20084-0) of the Republic of Korea.

Copyright 2009 Elsevier B.V., All rights reserved.


  • Gold nanoparticles
  • Homogenous nanogrowth
  • Magnetic separation
  • Protein biomarker
  • Solution phase

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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