Hot embossing for fabrication of a microfluidic 3D cell culture platform

Jessie S. Jeon; Seok Chung; Roger D. Kamm; Joseph L. Charest

doi:10.1007/s10544-010-9496-0

Hot embossing for fabrication of a microfluidic 3D cell culture platform

Jessie S. Jeon, Seok Chung, Roger D. Kamm, Joseph L. Charest

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

81 Citations (Scopus)

Abstract

Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microenvironment under controlled biochemical and biophysical parameters. In the present study, applying the known commercially-viable manufacturing methods to a cyclic olefin copolymer (COC) material resulted in a microfluidic device with enhanced 3D gel capabilities, controlled surface properties, and improved potential to serve high-volume applications. Hot embossing and roller lamination molded and sealed the microfluidic device. A combination of oxygen plasma and thermal treatments enhanced the sealing, ensured proper placement of the 3D gel, and created controlled and stable surface properties within the device. Culture of cells in the new device indicated no adverse effects of the COC material or processing as compared to previous PDMS devices. The results demonstrate a methodology to transition microfludic devices for 3D cell culture from scientific research to high-volume applications with broad clinical impact.

Original language	English
Pages (from-to)	325-333
Number of pages	9
Journal	Biomedical Microdevices
Volume	13
Issue number	2
DOIs	https://doi.org/10.1007/s10544-010-9496-0
Publication status	Published - 2011 Apr

Bibliographical note

Funding Information:
Acknowledgement The research is supported by Award Number R21CA140096 from the National Cancer Institute and Draper Laboratories Inc. (IR&D Grant). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institue of Health. Seok Chung was supported by the International Research & Development Program (Grant number: 2009-00631).

Keywords

3D cell culture
COC
Hot embossing
Microfluidics
Surface treatment
Thermal bonding

ASJC Scopus subject areas

Biomedical Engineering
Molecular Biology

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Cite this

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abstract = "Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microenvironment under controlled biochemical and biophysical parameters. In the present study, applying the known commercially-viable manufacturing methods to a cyclic olefin copolymer (COC) material resulted in a microfluidic device with enhanced 3D gel capabilities, controlled surface properties, and improved potential to serve high-volume applications. Hot embossing and roller lamination molded and sealed the microfluidic device. A combination of oxygen plasma and thermal treatments enhanced the sealing, ensured proper placement of the 3D gel, and created controlled and stable surface properties within the device. Culture of cells in the new device indicated no adverse effects of the COC material or processing as compared to previous PDMS devices. The results demonstrate a methodology to transition microfludic devices for 3D cell culture from scientific research to high-volume applications with broad clinical impact.",

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note = "Funding Information: Acknowledgement The research is supported by Award Number R21CA140096 from the National Cancer Institute and Draper Laboratories Inc. (IR&D Grant). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institue of Health. Seok Chung was supported by the International Research & Development Program (Grant number: 2009-00631).",

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Hot embossing for fabrication of a microfluidic 3D cell culture platform

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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