Human induced pluripotent stem cell line with cytochrome P450 enzyme polymorphism (CYP2C19*2/CYP3A5*3C) generated from lymphoblastoid cells

Jaehun Lee; Dong Hun Woo; Han Jin Park; Kijung Park; Duck Sung Ko; Jong Hoon Kim

doi:10.1016/j.scr.2017.12.014

Human induced pluripotent stem cell line with cytochrome P450 enzyme polymorphism (CYP2C192/CYP3A53C) generated from lymphoblastoid cells

Jaehun Lee, Dong Hun Woo, Han Jin Park, Kijung Park, Duck Sung Ko, Jong Hoon Kim

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Cytochrome P450 (CYP) comprises a superfamily of monooxygenase responsible for the metabolism of xenobiotics and approximately 75% of drugs in use today. Thus, genetic polymorphisms in CYP genes contribute to interindividual differences in hepatic metabolism of drugs, affecting on individual drug efficacy and may cause adverse effects. Here, we generated a human induced pluripotent stem cell (hiPSC) line with pharmacologically important traits (CYP2C19*2/CYP3A5*3C), which are highly polymorphic in Asian from lymphoblastoid cells. This hiPSC line could be a valuable source for predicting individual drug responses in the drug screening process that uses hiPSC-derived somatic cells, including hepatocytes.

Original language	English
Pages (from-to)	34-37
Number of pages	4
Journal	Stem Cell Research
Volume	27
DOIs	https://doi.org/10.1016/j.scr.2017.12.014
Publication status	Published - 2018 Mar

Bibliographical note

Funding Information:
We would like to thank Bom-Yi Lee and So-Yeon Park (Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center) for technical support in karyotype analysis. This study was provided with bioresources from National Biobank of Korea, the Centers for Disease Control and Prevention, Republic of Korea. This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2017M3A9B4042581 ).

Funding Information:
We would like to thank Bom-Yi Lee and So-Yeon Park (Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center) for technical support in karyotype analysis. This study was provided with bioresources from National Biobank of Korea, the Centers for Disease Control and Prevention, Republic of Korea. This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2017M3A9B4042581).

Publisher Copyright:
© 2017

ASJC Scopus subject areas

Developmental Biology
Cell Biology

Access to Document

10.1016/j.scr.2017.12.014

Cite this

@article{94adbca0a1544c79bf14eb1fcf99592f,

title = "Human induced pluripotent stem cell line with cytochrome P450 enzyme polymorphism (CYP2C19*2/CYP3A5*3C) generated from lymphoblastoid cells",

abstract = "Cytochrome P450 (CYP) comprises a superfamily of monooxygenase responsible for the metabolism of xenobiotics and approximately 75% of drugs in use today. Thus, genetic polymorphisms in CYP genes contribute to interindividual differences in hepatic metabolism of drugs, affecting on individual drug efficacy and may cause adverse effects. Here, we generated a human induced pluripotent stem cell (hiPSC) line with pharmacologically important traits (CYP2C19*2/CYP3A5*3C), which are highly polymorphic in Asian from lymphoblastoid cells. This hiPSC line could be a valuable source for predicting individual drug responses in the drug screening process that uses hiPSC-derived somatic cells, including hepatocytes.",

author = "Jaehun Lee and Woo, {Dong Hun} and Park, {Han Jin} and Kijung Park and Ko, {Duck Sung} and Kim, {Jong Hoon}",

note = "Funding Information: We would like to thank Bom-Yi Lee and So-Yeon Park (Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center) for technical support in karyotype analysis. This study was provided with bioresources from National Biobank of Korea, the Centers for Disease Control and Prevention, Republic of Korea. This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2017M3A9B4042581 ). Funding Information: We would like to thank Bom-Yi Lee and So-Yeon Park (Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center) for technical support in karyotype analysis. This study was provided with bioresources from National Biobank of Korea, the Centers for Disease Control and Prevention, Republic of Korea. This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MEST) (No. 2017M3A9B4042581). Publisher Copyright: {\textcopyright} 2017",

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AU - Park, Han Jin

AU - Park, Kijung

AU - Ko, Duck Sung

AU - Kim, Jong Hoon

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