Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

Chan Woo Kim; Tae Hoon Lee; Keun Hyung Park; Sang Yun Choi; Jiyoung Kim

doi:10.1016/j.febslet.2011.12.011

Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

Chan Woo Kim
, Tae Hoon Lee
, Keun Hyung Park
, Sang Yun Choi
, Jiyoung Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.

Original language	English
Pages (from-to)	229-234
Number of pages	6
Journal	FEBS Letters
Volume	586
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2011.12.011
Publication status	Published - 2012 Feb 3

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Education, Science and Technology) (No. R01-2008-000-20248-0 ) and Kyung Hee University Republic of Korea ( KHU-20100161 ).

Keywords

Endothelial cell
Inflammation
Intercellular adhesion molecule-1 (ICAM-1)
Lactoferrin
NF-κB

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2011.12.011

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title = "Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells",

abstract = "Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.",

keywords = "Endothelial cell, Inflammation, Intercellular adhesion molecule-1 (ICAM-1), Lactoferrin, NF-κB",

author = "Kim, \{Chan Woo\} and Lee, \{Tae Hoon\} and Park, \{Keun Hyung\} and Choi, \{Sang Yun\} and Jiyoung Kim",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Education, Science and Technology) (No. R01-2008-000-20248-0 ) and Kyung Hee University Republic of Korea ( KHU-20100161 ).",

year = "2012",

month = feb,

day = "3",

doi = "10.1016/j.febslet.2011.12.011",

language = "English",

volume = "586",

pages = "229--234",

journal = "FEBS Letters",

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T1 - Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

AU - Kim, Chan Woo

AU - Lee, Tae Hoon

AU - Park, Keun Hyung

AU - Choi, Sang Yun

AU - Kim, Jiyoung

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (Ministry of Education, Science and Technology) (No. R01-2008-000-20248-0 ) and Kyung Hee University Republic of Korea ( KHU-20100161 ).

PY - 2012/2/3

Y1 - 2012/2/3

N2 - Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.

AB - Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.

KW - Endothelial cell

KW - Inflammation

KW - Intercellular adhesion molecule-1 (ICAM-1)

KW - Lactoferrin

KW - NF-κB

UR - https://www.scopus.com/pages/publications/84862816950

U2 - 10.1016/j.febslet.2011.12.011

DO - 10.1016/j.febslet.2011.12.011

M3 - Article

C2 - 22226679

AN - SCOPUS:84862816950

SN - 0014-5793

VL - 586

SP - 229

EP - 234

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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