Human lactoferrin upregulates expression of KDR/Flk-1 and stimulates VEGF-A-mediated endothelial cell proliferation and migration

Chan Woo Kim; Kyung No Son; Sang Yun Choi; Jiyoung Kim

doi:10.1016/j.febslet.2006.06.091

Human lactoferrin upregulates expression of KDR/Flk-1 and stimulates VEGF-A-mediated endothelial cell proliferation and migration

Chan Woo Kim, Kyung No Son, Sang Yun Choi, Jiyoung Kim

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Lactoferrin (LF) is a multifunctional iron-binding glycoprotein, which plays a variety of biological processes including immunity. In this study, we demonstrate that human LF upregulates KDR/Flk-1 mRNA and protein levels in HUVECs at an optimal concentration of 5 μg/ml, which subsequently promotes the VEGF-induced proliferation and migration of the endothelial cells. Exposure of HUVECs to LF significantly increased VEGF-induced ERK MAP kinase phosphorylation. The maximal stimulation of KDR/Flk-1 expression by LF was correlated with LF-induced increase in cell proliferation and migration. These findings suggest that LF may stimulate in vivo angiogenesis via upregulation of KDR/Flk-1 expression in endothelial cells.

Original language	English
Pages (from-to)	4332-4336
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2006.06.091
Publication status	Published - 2006 Aug 7

Bibliographical note

Funding Information:
This work was supported by Grant No. R01-2004-000-10481-0 from the Basic Research Program of the Korea Science & Engineering Foundation.

Keywords

KDR/Flk-1
Lactoferrin
Migration
Proliferation
VEGF

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.06.091

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title = "Human lactoferrin upregulates expression of KDR/Flk-1 and stimulates VEGF-A-mediated endothelial cell proliferation and migration",

abstract = "Lactoferrin (LF) is a multifunctional iron-binding glycoprotein, which plays a variety of biological processes including immunity. In this study, we demonstrate that human LF upregulates KDR/Flk-1 mRNA and protein levels in HUVECs at an optimal concentration of 5 μg/ml, which subsequently promotes the VEGF-induced proliferation and migration of the endothelial cells. Exposure of HUVECs to LF significantly increased VEGF-induced ERK MAP kinase phosphorylation. The maximal stimulation of KDR/Flk-1 expression by LF was correlated with LF-induced increase in cell proliferation and migration. These findings suggest that LF may stimulate in vivo angiogenesis via upregulation of KDR/Flk-1 expression in endothelial cells.",

keywords = "KDR/Flk-1, Lactoferrin, Migration, Proliferation, VEGF",

author = "Kim, {Chan Woo} and Son, {Kyung No} and Choi, {Sang Yun} and Jiyoung Kim",

note = "Funding Information: This work was supported by Grant No. R01-2004-000-10481-0 from the Basic Research Program of the Korea Science & Engineering Foundation.",

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doi = "10.1016/j.febslet.2006.06.091",

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AU - Kim, Chan Woo

AU - Son, Kyung No

AU - Choi, Sang Yun

AU - Kim, Jiyoung

N1 - Funding Information: This work was supported by Grant No. R01-2004-000-10481-0 from the Basic Research Program of the Korea Science & Engineering Foundation.

PY - 2006/8/7

Y1 - 2006/8/7

N2 - Lactoferrin (LF) is a multifunctional iron-binding glycoprotein, which plays a variety of biological processes including immunity. In this study, we demonstrate that human LF upregulates KDR/Flk-1 mRNA and protein levels in HUVECs at an optimal concentration of 5 μg/ml, which subsequently promotes the VEGF-induced proliferation and migration of the endothelial cells. Exposure of HUVECs to LF significantly increased VEGF-induced ERK MAP kinase phosphorylation. The maximal stimulation of KDR/Flk-1 expression by LF was correlated with LF-induced increase in cell proliferation and migration. These findings suggest that LF may stimulate in vivo angiogenesis via upregulation of KDR/Flk-1 expression in endothelial cells.

AB - Lactoferrin (LF) is a multifunctional iron-binding glycoprotein, which plays a variety of biological processes including immunity. In this study, we demonstrate that human LF upregulates KDR/Flk-1 mRNA and protein levels in HUVECs at an optimal concentration of 5 μg/ml, which subsequently promotes the VEGF-induced proliferation and migration of the endothelial cells. Exposure of HUVECs to LF significantly increased VEGF-induced ERK MAP kinase phosphorylation. The maximal stimulation of KDR/Flk-1 expression by LF was correlated with LF-induced increase in cell proliferation and migration. These findings suggest that LF may stimulate in vivo angiogenesis via upregulation of KDR/Flk-1 expression in endothelial cells.

KW - KDR/Flk-1

KW - Lactoferrin

KW - Migration

KW - Proliferation

KW - VEGF

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DO - 10.1016/j.febslet.2006.06.091

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AN - SCOPUS:33746337305

SN - 0014-5793

VL - 580

SP - 4332

EP - 4336

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Human lactoferrin upregulates expression of KDR/Flk-1 and stimulates VEGF-A-mediated endothelial cell proliferation and migration

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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