Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death

Soo Hyun Choi; So Young Kim; Jae Jin An; Sun Hwa Lee; Dae Won Kim; Hea Jin Ryu; Nam Il Lee; Seung Il Yeo; Sang Ho Jang; Moo Ho Won; Tae Cheon Kang; Hyung Joo Kwon; Sung Woo Cho; Joon Kim; Kil Soo Lee; Jinseu Park; Won Sik Eum; Soo Young Choi

doi:10.1016/j.febslet.2006.11.038

Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death

Soo Hyun Choi, So Young Kim, Jae Jin An, Sun Hwa Lee, Dae Won Kim, Hea Jin Ryu, Nam Il Lee, Seung Il Yeo, Sang Ho Jang, Moo Ho Won, Tae Cheon Kang, Hyung Joo Kwon, Sung Woo Cho, Joon Kim, Kil Soo Lee, Jinseu Park, Won Sik Eum, Soo Young Choi

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48 h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.

Original language	English
Pages (from-to)	6755-6762
Number of pages	8
Journal	FEBS Letters
Volume	580
Issue number	30
DOIs	https://doi.org/10.1016/j.febslet.2006.11.038
Publication status	Published - 2006 Dec 22

Keywords

PEP-1 peptide
Protein therapy
Protein transduction
Ribosomal protein S3
Ultraviolet-damage

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2006.11.038

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Choi, S. H., Kim, S. Y., An, J. J., Lee, S. H., Kim, D. W., Ryu, H. J., Lee, N. I., Yeo, S. I., Jang, S. H., Won, M. H., Kang, T. C., Kwon, H. J., Cho, S. W., Kim, J., Lee, K. S., Park, J., Eum, W. S., & Choi, S. Y. (2006). Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death. FEBS Letters, 580(30), 6755-6762. https://doi.org/10.1016/j.febslet.2006.11.038

@article{3906e8a8505a4762a33b101be9c25ee8,

title = "Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death",

abstract = "The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48 h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.",

keywords = "PEP-1 peptide, Protein therapy, Protein transduction, Ribosomal protein S3, Ultraviolet-damage",

author = "Choi, {Soo Hyun} and Kim, {So Young} and An, {Jae Jin} and Lee, {Sun Hwa} and Kim, {Dae Won} and Ryu, {Hea Jin} and Lee, {Nam Il} and Yeo, {Seung Il} and Jang, {Sang Ho} and Won, {Moo Ho} and Kang, {Tae Cheon} and Kwon, {Hyung Joo} and Cho, {Sung Woo} and Joon Kim and Lee, {Kil Soo} and Jinseu Park and Eum, {Won Sik} and Choi, {Soo Young}",

note = "Funding Information: This work was supported by the Next Generation Growth Engine Program Grant (F104AC010002-06A0301-00210) and the Basic Research Grant (R01-2005-000-10798-0) from the Korean Science and Engineering Foundation and in part by the Basic Research Promotion Grant (KRF-2005-070-C00091) from Korea Research Foundation.",

year = "2006",

month = dec,

day = "22",

doi = "10.1016/j.febslet.2006.11.038",

language = "English",

volume = "580",

pages = "6755--6762",

journal = "FEBS Letters",

issn = "0014-5793",

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T1 - Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death

AU - Choi, Soo Hyun

AU - Kim, So Young

AU - An, Jae Jin

AU - Lee, Sun Hwa

AU - Kim, Dae Won

AU - Ryu, Hea Jin

AU - Lee, Nam Il

AU - Yeo, Seung Il

AU - Jang, Sang Ho

AU - Won, Moo Ho

AU - Kang, Tae Cheon

AU - Kwon, Hyung Joo

AU - Cho, Sung Woo

AU - Kim, Joon

AU - Lee, Kil Soo

AU - Park, Jinseu

AU - Eum, Won Sik

AU - Choi, Soo Young

N1 - Funding Information: This work was supported by the Next Generation Growth Engine Program Grant (F104AC010002-06A0301-00210) and the Basic Research Grant (R01-2005-000-10798-0) from the Korean Science and Engineering Foundation and in part by the Basic Research Promotion Grant (KRF-2005-070-C00091) from Korea Research Foundation.

PY - 2006/12/22

Y1 - 2006/12/22

N2 - The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48 h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.

AB - The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48 h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.

KW - PEP-1 peptide

KW - Protein therapy

KW - Protein transduction

KW - Ribosomal protein S3

KW - Ultraviolet-damage

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U2 - 10.1016/j.febslet.2006.11.038

DO - 10.1016/j.febslet.2006.11.038

M3 - Article

C2 - 17140567

AN - SCOPUS:33845419685

SN - 0014-5793

VL - 580

SP - 6755

EP - 6762

JO - FEBS Letters

JF - FEBS Letters

IS - 30

ER -

Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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