@article{1196ddda2cb74e418fe91c171202d4ad,
title = "HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions",
abstract = "All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.",
keywords = "39UTR, Polyadenylation signal, UVC, mRNA stability",
author = "Incheol Ryu and Yeonkyoung Park and Seo, {Jwa Won} and Park, {Ok Hyun} and Hongseok Ha and Nam, {Jin Wu} and Kim, {Yoon Ki}",
note = "Funding Information: The authors thank Dr. Berndt M{\"u}ller (University of Aberdeen, Aberdeen, United Kingdom) for providing the antibody against human SLBP, Dr. Lynne E. Maquat (University of Rochester, Rochester, NY, USA) for the antibody against human UPF1, and Dr. You-Sub Won (Korea University) for scientific comments. This work was supported by the National Research Foundation (NRF) of Korea funded by the Korean government (MSIP) (Grants NRF-2015R1A3A2033665 and NRF-2018R1A5A1024261 to Y.K.K.; and, in part, 2013R1A1A2063273 and 2016R1D1A1B3933894 to I.R.), and by a Korea University grant (to Y.K.K.). The authors declare no conflicts of interest. Funding Information: The authors thank Dr. Berndt M?ller (University of Aberdeen, Aberdeen, United Kingdom) for providing the antibody against human SLBP, Dr. Lynne E. Maquat (University of Rochester, Rochester, NY, USA) for the antibody against human UPF1, and Dr. You-Sub Won (Korea University) for scientific comments. This work was supported by the National Research Foundation (NRF) of Korea funded by the Korean government (MSIP) (Grants NRF-2015R1A3A2033665 and NRF-2018R1A5A1024261 to Y.K.K.; and, in part, 2013R1A1A2063273 and 2016R1D1A1B3933894 to I.R.), and by a Korea University grant (to Y.K.K.). The authors declare no conflicts of interest. Publisher Copyright: {\textcopyright} FASEB.",
year = "2019",
doi = "10.1096/fj.201800431R",
language = "English",
volume = "33",
pages = "2680--2693",
journal = "The FASEB journal : official publication of the Federation of American Societies for Experimental Biology",
issn = "1530-6860",
publisher = "FASEB",
number = "2",
}