Hydrocarbon production from secondarily treated piggery wastewater by the green alga Botryococcus braunii

Jin Young An, Sang Jun Sim, Jin Suk Lee, Byung Woo Kim

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174 Citations (Scopus)


A laboratory study was conducted on the removal of nitrogen and phosphorus from piggery wastewater during growth of Botryococcus braunii UTEX 572, together with measurements of hydrocarbon formation by the alga. The influence was tested of the initial nitrogen and phosphorus concentration on the optimum concentration range for a culture in secondarily treated piggery wastewater. A high cell density (> 7 g L-1 d. wt) was obtained with 510 mg L-1 NO3-N. Growth increased with nitrogen concentration at the basal phosphorus concentration (14 mg P L-1). The growth rate was nearly independent (μ = 0.027 ∼ 0.030 h-1) of the initial phosphate concentration, except under conditions of phosphate deficiency (μ = 0.019 h-1). B. braunii grew well in piggery wastewater pretreated by a membrane bioreactor (MBR) with acidogenic fermentation. A dry cell weight of 8.5 mg L-1 and hydrocarbon level of 0.95 g L-1 were obtained, and nitrate was removed at a rate of 620 mg N L-1. These results indicate that pretreated piggery wastewater provides a good culture medium for the growth and hydrocarbon production by B. braunii.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalJournal of Applied Phycology
Issue number2-3
Publication statusPublished - 2003 Mar
Externally publishedYes

Bibliographical note

Funding Information:
Financial support for this research was provided by a grant (00-J-CG-01-B-16-a1b1) from the Korea Ministry of Science and Technology.


  • Ammonium removal
  • Botryococcus braunii
  • Hydrocarbon
  • Nitrate removal
  • Phosphorus removal
  • Piggery wastewater

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science


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