Hydrodynamic stretching of single cells for high-throughput vector-free intracellular delivery of macromolecules

Yanxiang Deng, Megan Kizer, Xing Wang, Aram J. Chung

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We present a novel high-throughput vector-free macromolecule intracellular delivery microfluidic platform. The platform inertially focuses cells into the channel center and then guides them to be uniformly stretched via extensional flow near stagnation point. Hydrodynamic cell deformation creates nanopores on cellular and nuclear membranes that facilitate the transport of exogenous materials into the cell cytoplasm. The presented approach can effectively deliver a wide range of nanoscopic cargos to different cell types with high efficiency while maintaining high cell viability. This hydroporation method through cell stretching shows superior delivery efficiency, is high-throughput, simple, low-cost and clogging-free, and has high controllability.

    Original languageEnglish
    Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
    PublisherChemical and Biological Microsystems Society
    Pages1352-1354
    Number of pages3
    ISBN (Electronic)9781510897571
    Publication statusPublished - 2018 Jan 1
    Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
    Duration: 2018 Nov 112018 Nov 15

    Publication series

    Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
    Volume3

    Conference

    Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
    Country/TerritoryTaiwan, Province of China
    CityKaohsiung
    Period18/11/1118/11/15

    Keywords

    • Hydroporation
    • Inertial microfluidics
    • Intracellular delivery

    ASJC Scopus subject areas

    • General Chemistry
    • Bioengineering
    • Chemical Engineering (miscellaneous)
    • Control and Systems Engineering

    Fingerprint

    Dive into the research topics of 'Hydrodynamic stretching of single cells for high-throughput vector-free intracellular delivery of macromolecules'. Together they form a unique fingerprint.

    Cite this