Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid

Tam Anh D. Nguyen; Se Jong Han; Jun Pyo Kim; Mi Sun Kim; You Kwan Oh; Sang Jun Sim

doi:10.1016/j.ijhydene.2008.05.019

Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid

Tam Anh D. Nguyen, Se Jong Han, Jun Pyo Kim, Mi Sun Kim, You Kwan Oh, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

The application of thermophilic bacteria to the production of hydrogen (H₂) from a cellulosic biomass has captured attention of engineering researchers. Thermotoga neapolitana is considered as a potential hydrogen producer because of its ability to be used for the direct H₂ fermentation from raw cellulose, but its H₂ yield is not high. In this study, three chemical pretreatment methods with an ionic liquid, acid and alkali, respectively, were tested on cellulose to enhance the cellulose conversion of this strain into H₂. Batch cultivations were carried out to investigate the influence of the chemical pretreatment methods on H₂ production under non-sterile conditions. The highest H₂ concentration in the headspace obtained from the ionic liquid-pretreated cellulose was 36.1% (v/v) with ethanol as an anti-solvent. This was clearly higher than that from the acid- and alkali-pretreated celluloses (22-24%). Cultivation with cellulose pretreated with the ionic liquid at a concentration of 10% combining with N₂ sparging showed the maximum cumulative H₂ yield of 1280 mL H₂/L culture. This value is approximately 10-fold higher than that of raw cellulose (122 mL H₂/L culture). The H₂ yield obtained from fermentation with ionic liquid-pretreated cellulose was 2.2 mol H₂/mol glucose equivalents. The results showed that the pretreatment of cellulose using an ionic liquid has considerable potential for improving the direct conversion of cellulosic substrates into H₂ by bacteria.

Original language	English
Pages (from-to)	5161-5168
Number of pages	8
Journal	International Journal of Hydrogen Energy
Volume	33
Issue number	19
DOIs	https://doi.org/10.1016/j.ijhydene.2008.05.019
Publication status	Published - 2008 Oct
Externally published	Yes

Bibliographical note

Funding Information:
This Research was supported by the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea.

Keywords

Hydrogen production
Ionic liquid pretreatment
N sparging
Thermotoga neapolitana

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijhydene.2008.05.019

Cite this

@article{8d3c571ad0dd4ea1948ff3485f1bc954,

title = "Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid",

abstract = "The application of thermophilic bacteria to the production of hydrogen (H2) from a cellulosic biomass has captured attention of engineering researchers. Thermotoga neapolitana is considered as a potential hydrogen producer because of its ability to be used for the direct H2 fermentation from raw cellulose, but its H2 yield is not high. In this study, three chemical pretreatment methods with an ionic liquid, acid and alkali, respectively, were tested on cellulose to enhance the cellulose conversion of this strain into H2. Batch cultivations were carried out to investigate the influence of the chemical pretreatment methods on H2 production under non-sterile conditions. The highest H2 concentration in the headspace obtained from the ionic liquid-pretreated cellulose was 36.1% (v/v) with ethanol as an anti-solvent. This was clearly higher than that from the acid- and alkali-pretreated celluloses (22-24%). Cultivation with cellulose pretreated with the ionic liquid at a concentration of 10% combining with N2 sparging showed the maximum cumulative H2 yield of 1280 mL H2/L culture. This value is approximately 10-fold higher than that of raw cellulose (122 mL H2/L culture). The H2 yield obtained from fermentation with ionic liquid-pretreated cellulose was 2.2 mol H2/mol glucose equivalents. The results showed that the pretreatment of cellulose using an ionic liquid has considerable potential for improving the direct conversion of cellulosic substrates into H2 by bacteria.",

keywords = "Hydrogen production, Ionic liquid pretreatment, N sparging, Thermotoga neapolitana",

author = "Nguyen, {Tam Anh D.} and Han, {Se Jong} and Kim, {Jun Pyo} and Kim, {Mi Sun} and Oh, {You Kwan} and Sim, {Sang Jun}",

note = "Funding Information: This Research was supported by the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea.",

year = "2008",

month = oct,

doi = "10.1016/j.ijhydene.2008.05.019",

language = "English",

volume = "33",

pages = "5161--5168",

journal = "International Journal of Hydrogen Energy",

issn = "0360-3199",

publisher = "Elsevier Ltd",

number = "19",

}

TY - JOUR

T1 - Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid

AU - Nguyen, Tam Anh D.

AU - Han, Se Jong

AU - Kim, Jun Pyo

AU - Kim, Mi Sun

AU - Oh, You Kwan

AU - Sim, Sang Jun

N1 - Funding Information: This Research was supported by the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea.

PY - 2008/10

Y1 - 2008/10

N2 - The application of thermophilic bacteria to the production of hydrogen (H2) from a cellulosic biomass has captured attention of engineering researchers. Thermotoga neapolitana is considered as a potential hydrogen producer because of its ability to be used for the direct H2 fermentation from raw cellulose, but its H2 yield is not high. In this study, three chemical pretreatment methods with an ionic liquid, acid and alkali, respectively, were tested on cellulose to enhance the cellulose conversion of this strain into H2. Batch cultivations were carried out to investigate the influence of the chemical pretreatment methods on H2 production under non-sterile conditions. The highest H2 concentration in the headspace obtained from the ionic liquid-pretreated cellulose was 36.1% (v/v) with ethanol as an anti-solvent. This was clearly higher than that from the acid- and alkali-pretreated celluloses (22-24%). Cultivation with cellulose pretreated with the ionic liquid at a concentration of 10% combining with N2 sparging showed the maximum cumulative H2 yield of 1280 mL H2/L culture. This value is approximately 10-fold higher than that of raw cellulose (122 mL H2/L culture). The H2 yield obtained from fermentation with ionic liquid-pretreated cellulose was 2.2 mol H2/mol glucose equivalents. The results showed that the pretreatment of cellulose using an ionic liquid has considerable potential for improving the direct conversion of cellulosic substrates into H2 by bacteria.

AB - The application of thermophilic bacteria to the production of hydrogen (H2) from a cellulosic biomass has captured attention of engineering researchers. Thermotoga neapolitana is considered as a potential hydrogen producer because of its ability to be used for the direct H2 fermentation from raw cellulose, but its H2 yield is not high. In this study, three chemical pretreatment methods with an ionic liquid, acid and alkali, respectively, were tested on cellulose to enhance the cellulose conversion of this strain into H2. Batch cultivations were carried out to investigate the influence of the chemical pretreatment methods on H2 production under non-sterile conditions. The highest H2 concentration in the headspace obtained from the ionic liquid-pretreated cellulose was 36.1% (v/v) with ethanol as an anti-solvent. This was clearly higher than that from the acid- and alkali-pretreated celluloses (22-24%). Cultivation with cellulose pretreated with the ionic liquid at a concentration of 10% combining with N2 sparging showed the maximum cumulative H2 yield of 1280 mL H2/L culture. This value is approximately 10-fold higher than that of raw cellulose (122 mL H2/L culture). The H2 yield obtained from fermentation with ionic liquid-pretreated cellulose was 2.2 mol H2/mol glucose equivalents. The results showed that the pretreatment of cellulose using an ionic liquid has considerable potential for improving the direct conversion of cellulosic substrates into H2 by bacteria.

KW - Hydrogen production

KW - Ionic liquid pretreatment

KW - N sparging

KW - Thermotoga neapolitana

UR - http://www.scopus.com/inward/record.url?scp=52049088253&partnerID=8YFLogxK

U2 - 10.1016/j.ijhydene.2008.05.019

DO - 10.1016/j.ijhydene.2008.05.019

M3 - Article

AN - SCOPUS:52049088253

SN - 0360-3199

VL - 33

SP - 5161

EP - 5168

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 19

ER -

Hydrogen production by the hyperthermophilic eubacterium, Thermotoga neapolitana, using cellulose pretreated by ionic liquid

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this