TY - JOUR
T1 - Hydrogenophaga crassostreae sp. nov., isolated from a Pacific oyster
AU - Baek, Chaeyun
AU - Kim, Eunji
AU - Shin, Su Kyoung
AU - Choi, Sungmi
AU - Yi, Hana
N1 - Funding Information:
This study was supported by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources, funded by the Korean Ministry of Environment, the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education (2015R1D1A1A01057527), and the National Research Council of Science and Technology (NST) grant by the Korean government (MSIP) (No. CRC-16–01-KRICT).
Publisher Copyright:
© 2017 IUMS.
PY - 2017/10
Y1 - 2017/10
N2 - A Gram-negative, motile, rod-shaped, and aerobic bacterial strain, designated LPB0072T, was isolated from a Pacific oyster (Crassostrea gigas). Autotrophic growth with hydrogen gas was not observed. Cells oxidized thiosulfate to sulfate and reduced nitrate to nitrite. The complete genome sequence of strain LPB0072T (CP017476) is 4.94 Mb in length and contains 4459 protein-coding genes, with a G+C content of 61.3 mol%. Analysis of the 16S rRNA gene sequence indicated that strain LPB0072T belongs to the genus Hydrogenophaga, with greatest sequence similarity to the type strain of Hydrogenophaga taeniospiralis (97.5 %). The isoprenoid quinone (Q-8) and the major cellular fatty acids (C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0 and C17 : 1ω6c) identified were concordant with the chemotaxonomic properties of the genus Hydrogenophaga. The average nucleotide identities with closely related species were below the suggested boundary for species delineation, indicating that the isolate is a novel species. Numerous physiological and biochemical features also distinguished the isolate from other known Hydrogenophaga species. Based on the polyphasic data presented in this study, strain LPB0072T should be classified as a novel species in the genus Hydrogenophaga, and the name Hydrogenophaga crassostreae sp. nov. is proposed. The type strain is LPB0072T (=KACC 18705T=JCM 31188T).
AB - A Gram-negative, motile, rod-shaped, and aerobic bacterial strain, designated LPB0072T, was isolated from a Pacific oyster (Crassostrea gigas). Autotrophic growth with hydrogen gas was not observed. Cells oxidized thiosulfate to sulfate and reduced nitrate to nitrite. The complete genome sequence of strain LPB0072T (CP017476) is 4.94 Mb in length and contains 4459 protein-coding genes, with a G+C content of 61.3 mol%. Analysis of the 16S rRNA gene sequence indicated that strain LPB0072T belongs to the genus Hydrogenophaga, with greatest sequence similarity to the type strain of Hydrogenophaga taeniospiralis (97.5 %). The isoprenoid quinone (Q-8) and the major cellular fatty acids (C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0 and C17 : 1ω6c) identified were concordant with the chemotaxonomic properties of the genus Hydrogenophaga. The average nucleotide identities with closely related species were below the suggested boundary for species delineation, indicating that the isolate is a novel species. Numerous physiological and biochemical features also distinguished the isolate from other known Hydrogenophaga species. Based on the polyphasic data presented in this study, strain LPB0072T should be classified as a novel species in the genus Hydrogenophaga, and the name Hydrogenophaga crassostreae sp. nov. is proposed. The type strain is LPB0072T (=KACC 18705T=JCM 31188T).
KW - Novel species
KW - Pacific oyster
KW - Sequence analysis
UR - http://www.scopus.com/inward/record.url?scp=85031814004&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002244
DO - 10.1099/ijsem.0.002244
M3 - Article
C2 - 28901906
AN - SCOPUS:85031814004
SN - 1466-5026
VL - 67
SP - 4045
EP - 4049
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
M1 - 002244
ER -