Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans

Sang Duck Jeon; Su Jung Kim; Sung Hyun Park; Gi Wook Choi; Sung Ok Han

doi:10.1016/j.biortech.2015.02.071

Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans

Sang Duck Jeon, Su Jung Kim, Sung Hyun Park, Gi Wook Choi, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.

Original language	English
Pages (from-to)	505-511
Number of pages	7
Journal	Bioresource technology
Volume	191
DOIs	https://doi.org/10.1016/j.biortech.2015.02.071
Publication status	Published - 2015 Sept

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd

Keywords

Cellulose hydrolysis
Cellulosome
Clostridium cellulovorans
Scaffolding protein
Synergistic effect

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2015.02.071

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title = "Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans",

abstract = "The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.",

keywords = "Cellulose hydrolysis, Cellulosome, Clostridium cellulovorans, Scaffolding protein, Synergistic effect",

author = "Jeon, {Sang Duck} and Kim, {Su Jung} and Park, {Sung Hyun} and Choi, {Gi Wook} and Han, {Sung Ok}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd",

year = "2015",

month = sep,

doi = "10.1016/j.biortech.2015.02.071",

language = "English",

volume = "191",

pages = "505--511",

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T1 - Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans

AU - Jeon, Sang Duck

AU - Kim, Su Jung

AU - Park, Sung Hyun

AU - Choi, Gi Wook

AU - Han, Sung Ok

PY - 2015/9

Y1 - 2015/9

N2 - The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.

AB - The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.

KW - Cellulose hydrolysis

KW - Cellulosome

KW - Clostridium cellulovorans

KW - Scaffolding protein

KW - Synergistic effect

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SN - 0960-8524

VL - 191

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JO - Bioresource technology

JF - Bioresource technology

ER -

Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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