Hypocholesterolemic and hypoglycemic effects of enzymatically modified carbohydrates from rice in high-fat-fed C57BL/6J mice

Muhammad J.H. Bhuiyan, Ha Viet Do, Saehun Mun, Hee jin Jun, Ji Hae Lee, Yong Ro Kim, Sung Joon Lee

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Scope: Enzymatically modified rice starch (ERS) synthesized with 4-α-glucanotransferase has a longer structure than rice starch, which could delay digestion, similar to dietary fiber. We investigated the effects of ERS on glucose and lipid metabolism with mice fed a high-fat diet containing ERS (HFD-ERS). Method and results: Four weeks of ERS feeding showed hypoglycemic effects with a significant reduction in fasting glucose (46%), insulin (57%), and leptin (83%) levels; improved glucose tolerance (20% in AUC of oral glucose tolerance test); and increased adiponectin concentrations (+27%) compared to the HFD group. Notably, phosphorylation of AMP kinase (AMPK) was markedly induced in the HFD-ERS livers compared to HFD livers. Additionally, ERS significantly reduced total cholesterol concentrations with induction of fecal bile acid excretion (+21%, P<0.05) in the HFD-ERS group compared to the HFD group. The mRNA and protein expressions of hepatic LDL receptors were significantly induced. However, cholesterol 7 alpha-hydroxylase (CYP7A1) expression was downregulated possibly due to induction of intestinal farnesoid X receptor (FXR; +2.4-fold, p<0.05) and fibroblast growth factor-15 (FGF-15; +2.2-fold, p<0.01). Conclusion: Our data suggest that ERS feeding may have hypoglycemic and hypocholesterolemic effects via a mechanism similar to that of dietary fiber.

Original languageEnglish
Pages (from-to)S214-S226
JournalMolecular Nutrition and Food Research
Issue numberSUPPL. 2
Publication statusPublished - 2011 Sept


  • Bile acid excretion
  • Cholesterol
  • Glucose metabolism
  • Lipid metabolism
  • Rice starch

ASJC Scopus subject areas

  • Biotechnology
  • Food Science


Dive into the research topics of 'Hypocholesterolemic and hypoglycemic effects of enzymatically modified carbohydrates from rice in high-fat-fed C57BL/6J mice'. Together they form a unique fingerprint.

Cite this