Identification and analysis of a differentially expressed wheat RING-type E3 ligase in spike primordia development during post-vernalization

Jae Ho Kim, Irfan Ullah Khan, Cheol Won Lee, Dae Yeon Kim, Cheol Seong Jang, Sung Don Lim, Yong Chan Park, Ju Hee Kim, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Key message: We identified a RING-type E3 ligase (TaBAH1) protein in winter wheat that targets TaSAHH1 for degradation and might be involved in primordia development by regulating targeted protein degradation. Abstract: Grain yield per spike in wheat (Triticum aestivum), is mainly determined prior to flowering during mature primordia development; however, the genes involved in primordia development have yet to be characterized. In this study, we demonstrated that, after vernalization for 50 days at 4 °C, there was a rapid acceleration in primordia development to the mature stages in the winter wheat cultivars Keumgang and Yeongkwang compared with the Chinese Spring cultivar. Although Yeongkwang flowers later than Keumgang under normal condition, it has the same heading time and reaches the WS9 stage of floral development after vernalization for 50 days. Using RNA sequencing, we identified candidate genes associated with primordia development in cvs. Keumgang and Yeongkwang, that are differentially expressed during wheat reproductive stages. Among these, the RING-type E3 ligase TaBAH1 (TraesCS5B01G373000) was transcriptionally upregulated between the double-ridge (WS2.5) stage and later stages of floret primordia development (WS10) after vernalization. Transient expression analysis indicated that TaBAH1 was localized to the plasma membrane and nucleus and was characterized by self-ubiquitination activity. Furthermore, we found that TaBAH1 interacts with TaSAHH1 to mediate its polyubiquitination and degradation through a 26S proteasomal pathway. Collectively, the findings of this study indicate that TaBAH1 might play a prominent role in post-vernalization floret primordia development.

Original languageEnglish
Pages (from-to)543-558
Number of pages16
JournalPlant Cell Reports
Issue number3
Publication statusPublished - 2021 Mar

Bibliographical note

Funding Information:
This work was supported by a Korea University Grant and Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (2017R1D1A1B06030349).

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.


  • Primordia development
  • RING-type E3 ligase
  • Vernalization
  • Waddington scale
  • Wheat (triticum aestivum)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


Dive into the research topics of 'Identification and analysis of a differentially expressed wheat RING-type E3 ligase in spike primordia development during post-vernalization'. Together they form a unique fingerprint.

Cite this