Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum

Hyun Young Cho; Seong Gyu Lee; Jeong Eun Hyeon; Sung Ok Han

doi:10.1016/j.bbrc.2010.09.057

Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum

Hyun Young Cho, Seong Gyu Lee, Jeong Eun Hyeon, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We have identified and characterized a novel transcriptional regulator that binds to the promoter region of succinyl-CoA synthetase (sucCD) in Corynebacterium glutamicum. Using biotin-labeled DNA affinity beads, we identified a DeoR-type transcriptional regulator, SucR (Cg0146), which is a protein consisting of 282 amino acids with a mass of 31 kDa and RamB (Cg0444). The results of electrophoretic mobility shift assays verified that these regulators specifically bind to the sucCD promoter region. The putative SucR binding region extends from position -155 to -146 (a 10 bp sequence, ACTCTAGGGG) relative to the transcriptional start point of the sucCD operon. The expression level of sucCD in a sucR deletion mutant was seven times higher than that in wild-type cells grown on acetate. The increase in succinyl-CoA synthetase levels caused by inactivation of sucR. These assays revealed that SucR acts as a repressor of sucCD expression during acetate metabolism.

Original language	English
Pages (from-to)	300-305
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	401
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.09.057
Publication status	Published - 2010 Oct 15

Bibliographical note

Funding Information:
We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This work was supported in part by a National Research Foundation of Korea Grant funded by the Korean Government ( 2009-0073856 ) and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (No. M102KK010014-08K1101-01421 ).

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Keywords

Acetate metabolism
Corynebacterium glutamicum
SucCD expression
Succinyl-CoA synthetase
Transcriptional regulator

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2010.09.057

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title = "Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum",

abstract = "We have identified and characterized a novel transcriptional regulator that binds to the promoter region of succinyl-CoA synthetase (sucCD) in Corynebacterium glutamicum. Using biotin-labeled DNA affinity beads, we identified a DeoR-type transcriptional regulator, SucR (Cg0146), which is a protein consisting of 282 amino acids with a mass of 31 kDa and RamB (Cg0444). The results of electrophoretic mobility shift assays verified that these regulators specifically bind to the sucCD promoter region. The putative SucR binding region extends from position -155 to -146 (a 10 bp sequence, ACTCTAGGGG) relative to the transcriptional start point of the sucCD operon. The expression level of sucCD in a sucR deletion mutant was seven times higher than that in wild-type cells grown on acetate. The increase in succinyl-CoA synthetase levels caused by inactivation of sucR. These assays revealed that SucR acts as a repressor of sucCD expression during acetate metabolism.",

keywords = "Acetate metabolism, Corynebacterium glutamicum, SucCD expression, Succinyl-CoA synthetase, Transcriptional regulator",

author = "Cho, {Hyun Young} and Lee, {Seong Gyu} and Hyeon, {Jeong Eun} and Han, {Sung Ok}",

note = "Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This work was supported in part by a National Research Foundation of Korea Grant funded by the Korean Government ( 2009-0073856 ) and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (No. M102KK010014-08K1101-01421 ). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

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AU - Cho, Hyun Young

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AU - Hyeon, Jeong Eun

AU - Han, Sung Ok

N1 - Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This work was supported in part by a National Research Foundation of Korea Grant funded by the Korean Government ( 2009-0073856 ) and the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea Government (MOST) (No. M102KK010014-08K1101-01421 ). Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2010/10/15

Y1 - 2010/10/15

N2 - We have identified and characterized a novel transcriptional regulator that binds to the promoter region of succinyl-CoA synthetase (sucCD) in Corynebacterium glutamicum. Using biotin-labeled DNA affinity beads, we identified a DeoR-type transcriptional regulator, SucR (Cg0146), which is a protein consisting of 282 amino acids with a mass of 31 kDa and RamB (Cg0444). The results of electrophoretic mobility shift assays verified that these regulators specifically bind to the sucCD promoter region. The putative SucR binding region extends from position -155 to -146 (a 10 bp sequence, ACTCTAGGGG) relative to the transcriptional start point of the sucCD operon. The expression level of sucCD in a sucR deletion mutant was seven times higher than that in wild-type cells grown on acetate. The increase in succinyl-CoA synthetase levels caused by inactivation of sucR. These assays revealed that SucR acts as a repressor of sucCD expression during acetate metabolism.

AB - We have identified and characterized a novel transcriptional regulator that binds to the promoter region of succinyl-CoA synthetase (sucCD) in Corynebacterium glutamicum. Using biotin-labeled DNA affinity beads, we identified a DeoR-type transcriptional regulator, SucR (Cg0146), which is a protein consisting of 282 amino acids with a mass of 31 kDa and RamB (Cg0444). The results of electrophoretic mobility shift assays verified that these regulators specifically bind to the sucCD promoter region. The putative SucR binding region extends from position -155 to -146 (a 10 bp sequence, ACTCTAGGGG) relative to the transcriptional start point of the sucCD operon. The expression level of sucCD in a sucR deletion mutant was seven times higher than that in wild-type cells grown on acetate. The increase in succinyl-CoA synthetase levels caused by inactivation of sucR. These assays revealed that SucR acts as a repressor of sucCD expression during acetate metabolism.

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Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum

Abstract

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Keywords

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