NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.
|Number of pages
|Biochemical and biophysical research communications
|Published - 2010 Jan 1
Bibliographical noteFunding Information:
This work was supported by the Basic Research Program of the Korea Science and Engineering Foundation ( R13-2005-012-02002-0 ) and a Korea Research Foundation Grant ( KRF-2006-005-J04204 ). The nucleotide sequences reported in this paper have been submitted to the GenBank_databases under Accession No. GQ376510.
- Alternative splicing
- Neurite outgrowth
- Truncated NELL2
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology