Identification and characterization of new microRNAs from pig

Jung Kim; Ik Sang Cho; Jae Sang Hong; Young Ki Choi; Hyunggee Kim; Young Sik Lee

doi:10.1007/s00335-008-9111-3

Identification and characterization of new microRNAs from pig

Jung Kim, Ik Sang Cho, Jae Sang Hong, Young Ki Choi, Hyunggee Kim, Young Sik Lee

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42 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.

Original language	English
Pages (from-to)	570-580
Number of pages	11
Journal	Mammalian Genome
Volume	19
Issue number	7-8
DOIs	https://doi.org/10.1007/s00335-008-9111-3
Publication status	Published - 2008 Aug

ASJC Scopus subject areas

Genetics

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10.1007/s00335-008-9111-3

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title = "Identification and characterization of new microRNAs from pig",

abstract = "MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.",

author = "Jung Kim and Cho, {Ik Sang} and Hong, {Jae Sang} and Choi, {Young Ki} and Hyunggee Kim and Lee, {Young Sik}",

note = "Funding Information: This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.",

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T1 - Identification and characterization of new microRNAs from pig

AU - Kim, Jung

AU - Cho, Ik Sang

AU - Hong, Jae Sang

AU - Choi, Young Ki

AU - Kim, Hyunggee

AU - Lee, Young Sik

N1 - Funding Information: This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

PY - 2008/8

Y1 - 2008/8

N2 - MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.

AB - MicroRNAs (miRNAs) are small regulatory RNAs that direct the posttranscriptional repression of cognate messenger RNAs. Despite increasing evidence for diverse roles of miRNAs in biological processes, little is known about miRNAs in pig. We describe the first experimental identification of porcine miRNAs by sequence analysis of a cDNA library of small RNAs from porcine fibroblast cells. We identified 25 distinct porcine miRNAs, of which 19 are previously unreported, and define 14 new miRNA families in pig. Most of the cloned miRNAs are expressed ubiquitously in all porcine tissues examined, whereas some miRNAs are expressed preferentially in specific tissues. Our results enrich the porcine miRNA database and provide useful information for investigating biological functions of miRNAs in pig.

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Identification and characterization of new microRNAs from pig

Abstract

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