Identification and characterization of RNF2 response elements in human kidney cells

Dongwon Choi; Seongman Kang

doi:10.1007/s10059-011-0033-7

Identification and characterization of RNF2 response elements in human kidney cells

Dongwon Choi, Seongman Kang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

Original language	English
Pages (from-to)	247-253
Number of pages	7
Journal	Molecules and cells
Volume	31
Issue number	3
DOIs	https://doi.org/10.1007/s10059-011-0033-7
Publication status	Published - 2011 Mar

Bibliographical note

Funding Information:
This work was supported by grants from the National Research Foundation of Korea [C00216]; the Korea Research Foundation [2009-0074201].

Keywords

PcG complex
RNF2
RNF2-responding loci

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1007/s10059-011-0033-7

Cite this

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title = "Identification and characterization of RNF2 response elements in human kidney cells",

abstract = "RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.",

keywords = "PcG complex, RNF2, RNF2-responding loci",

author = "Dongwon Choi and Seongman Kang",

note = "Funding Information: This work was supported by grants from the National Research Foundation of Korea [C00216]; the Korea Research Foundation [2009-0074201].",

year = "2011",

month = mar,

doi = "10.1007/s10059-011-0033-7",

language = "English",

volume = "31",

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T1 - Identification and characterization of RNF2 response elements in human kidney cells

AU - Choi, Dongwon

AU - Kang, Seongman

N1 - Funding Information: This work was supported by grants from the National Research Foundation of Korea [C00216]; the Korea Research Foundation [2009-0074201].

PY - 2011/3

Y1 - 2011/3

N2 - RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

AB - RING finger protein 2 (RNF2) contains a conserved N-terminal RING finger domain and functions as an E3 ligase. As a member of the Polycomb group family of proteins, RNF2 also represses a number of genes involved in development, differentiation, malignant transformation and cell cycle. Herein, using chromatin immunoprecipitation cloning, 33 RNF2-responding loci were identified in the genome of HEK293 human kidney cells. Luciferase reporter assays showed that among them, 26 and 2 loci acted as a repressor and an activator, respectively. RNA interference revealed that the identified RNF2-responding sequences regulated the transcriptional activity of nearby promoters. This study may contribute to elucidating the mechanism underlying RNF2-mediated transcriptional regulation.

KW - PcG complex

KW - RNF2

KW - RNF2-responding loci

UR - https://www.scopus.com/pages/publications/79959936616

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DO - 10.1007/s10059-011-0033-7

M3 - Article

C2 - 21347701

AN - SCOPUS:79959936616

SN - 1016-8478

VL - 31

SP - 247

EP - 253

JO - Molecules and cells

JF - Molecules and cells

IS - 3

ER -

Identification and characterization of RNF2 response elements in human kidney cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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