Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

Jae Wook Yoon; Jae Yeon Jung; Hyun Jung Chung; Mi Ryung Kim; Chan Wha Kim; Seung Taik Lim

doi:10.1016/j.jcs.2010.06.015

Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

Jae Wook Yoon
, Jae Yeon Jung
, Hyun Jung Chung
, Mi Ryung Kim
, Chan Wha Kim
, Seung Taik Lim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products.

Original language	English
Pages (from-to)	321-326
Number of pages	6
Journal	Journal of Cereal Science
Volume	52
Issue number	2
DOIs	https://doi.org/10.1016/j.jcs.2010.06.015
Publication status	Published - 2010 Sept

Keywords

Botanical origin
SDS-PAGE
Starch granule-associated protein (SGAP)
Starch noodle

ASJC Scopus subject areas

Food Science
Biochemistry

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10.1016/j.jcs.2010.06.015

Cite this

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title = "Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins",

abstract = "Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products.",

keywords = "Botanical origin, SDS-PAGE, Starch granule-associated protein (SGAP), Starch noodle",

author = "Yoon, \{Jae Wook\} and Jung, \{Jae Yeon\} and Chung, \{Hyun Jung\} and Kim, \{Mi Ryung\} and Kim, \{Chan Wha\} and Lim, \{Seung Taik\}",

year = "2010",

month = sep,

doi = "10.1016/j.jcs.2010.06.015",

language = "English",

volume = "52",

pages = "321--326",

journal = "Journal of Cereal Science",

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publisher = "Academic Press",

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TY - JOUR

T1 - Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

AU - Yoon, Jae Wook

AU - Jung, Jae Yeon

AU - Chung, Hyun Jung

AU - Kim, Mi Ryung

AU - Kim, Chan Wha

AU - Lim, Seung Taik

PY - 2010/9

Y1 - 2010/9

N2 - Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products.

AB - Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products.

KW - Botanical origin

KW - SDS-PAGE

KW - Starch granule-associated protein (SGAP)

KW - Starch noodle

UR - https://www.scopus.com/pages/publications/77956441717

U2 - 10.1016/j.jcs.2010.06.015

DO - 10.1016/j.jcs.2010.06.015

M3 - Article

AN - SCOPUS:77956441717

SN - 0733-5210

VL - 52

SP - 321

EP - 326

JO - Journal of Cereal Science

JF - Journal of Cereal Science

IS - 2

ER -

Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

Abstract

Keywords

ASJC Scopus subject areas

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