Abstract
Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown-red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observed in each of the lines 142-3, 421-2, and 1376-1. To identify causal mutations for the seed coat colour change, the three mutant lines and the wild type were subjected to whole-genome re-sequencing. After removing natural variations, 906, 1057, and 978 DNA polymorphisms were detected in 142-3, 421-2, and 1376-1, respectively. A total of 13 high-risk DNA polymorphisms were identified in mutant 142-3. Based on a comparison with DNA polymorphisms in 421-2 and 1376-1, candidate causal mutations for the seed coat colour change in 142-3 were selected. In the two independent Arabidopsis thaliana lines carrying T-DNA insertions in the AtCHI, seed colour change was observed. We propose a frameshift mutation in BdCHI1 as a causal mutation responsible for seed colour change in 142-3. The DNA polymorphism information for these mutant lines can be utilized for functional genomics in B. distachyon and cereal crops.
Original language | English |
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Pages (from-to) | 581-587 |
Number of pages | 7 |
Journal | Genome |
Volume | 60 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2017 |
Bibliographical note
Publisher Copyright:© 2017 Published by NRC Research Press.
Keywords
- Brachypodium distachyon
- Gamma radiation
- InDel
- SNP
- Seed coat colour
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Genetics