Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing

Man Bo Lee, Dae Yeon Kim, Yong Weon Seo, F. Belzile

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown-red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observed in each of the lines 142-3, 421-2, and 1376-1. To identify causal mutations for the seed coat colour change, the three mutant lines and the wild type were subjected to whole-genome re-sequencing. After removing natural variations, 906, 1057, and 978 DNA polymorphisms were detected in 142-3, 421-2, and 1376-1, respectively. A total of 13 high-risk DNA polymorphisms were identified in mutant 142-3. Based on a comparison with DNA polymorphisms in 421-2 and 1376-1, candidate causal mutations for the seed coat colour change in 142-3 were selected. In the two independent Arabidopsis thaliana lines carrying T-DNA insertions in the AtCHI, seed colour change was observed. We propose a frameshift mutation in BdCHI1 as a causal mutation responsible for seed colour change in 142-3. The DNA polymorphism information for these mutant lines can be utilized for functional genomics in B. distachyon and cereal crops.

    Original languageEnglish
    Pages (from-to)581-587
    Number of pages7
    JournalGenome
    Volume60
    Issue number7
    DOIs
    Publication statusPublished - 2017

    Bibliographical note

    Publisher Copyright:
    © 2017 Published by NRC Research Press.

    Keywords

    • Brachypodium distachyon
    • Gamma radiation
    • InDel
    • SNP
    • Seed coat colour

    ASJC Scopus subject areas

    • Biotechnology
    • Molecular Biology
    • Genetics

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