Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage

S. Ok; Y. S. Chung; B. Y. Um; M. S. Park; J. M. Bae; S. J. Lee; J. S. Shin

doi:10.1007/s002990000211

Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage

S. Ok, Y. S. Chung, B. Y. Um, M. S. Park, J. M. Bae, S. J. Lee, J. S. Shin

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7%) revealed a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome, random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.

Original language	English
Pages (from-to)	932-937
Number of pages	6
Journal	Plant Cell Reports
Volume	19
Issue number	9
DOIs	https://doi.org/10.1007/s002990000211
Publication status	Published - 2000

Keywords

CDNA sequence
Citrullus lanatus
Expressed sequence tags
Watermelon

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s002990000211

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@article{1007a06a483647748075795f6f95c97f,

title = "Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage",

abstract = "A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7%) revealed a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome, random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.",

keywords = "CDNA sequence, Citrullus lanatus, Expressed sequence tags, Watermelon",

author = "S. Ok and Chung, {Y. S.} and Um, {B. Y.} and Park, {M. S.} and Bae, {J. M.} and Lee, {S. J.} and Shin, {J. S.}",

note = "Funding Information: Acknowledgements We thank Dr. Sangduk Kim for her valuable comments on this manuscript. This research was funded by the MAF-SGRF (Ministry of Agriculture and Forestry Special Grants Research Program) and partially supported by a grant from Korea University.",

year = "2000",

doi = "10.1007/s002990000211",

language = "English",

volume = "19",

pages = "932--937",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "9",

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TY - JOUR

T1 - Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage

AU - Ok, S.

AU - Chung, Y. S.

AU - Um, B. Y.

AU - Park, M. S.

AU - Bae, J. M.

AU - Lee, S. J.

AU - Shin, J. S.

N1 - Funding Information: Acknowledgements We thank Dr. Sangduk Kim for her valuable comments on this manuscript. This research was funded by the MAF-SGRF (Ministry of Agriculture and Forestry Special Grants Research Program) and partially supported by a grant from Korea University.

PY - 2000

Y1 - 2000

N2 - A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7%) revealed a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome, random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.

AB - A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7%) revealed a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome, random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.

KW - CDNA sequence

KW - Citrullus lanatus

KW - Expressed sequence tags

KW - Watermelon

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U2 - 10.1007/s002990000211

DO - 10.1007/s002990000211

M3 - Article

AN - SCOPUS:0033796356

SN - 0721-7714

VL - 19

SP - 932

EP - 937

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 9

ER -

Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage

Abstract

Keywords

ASJC Scopus subject areas

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