Abstract
2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
Original language | English |
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Pages (from-to) | 659-667 |
Number of pages | 9 |
Journal | Journal of microbiology and biotechnology |
Volume | 22 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2012 May |
Keywords
- 2,3-butanediol fermentation
- Continuous culture
- Intracellular metabolic measurements
- Metabolic flux analysis
- pH influence
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology