Abstract
To identify genes related to initiation of storage root development in sweetpotato, a cDNA library was constructed with early stage storage roots (0.3-1 cm in diameter). Single-pass sequences of the 5′ ends of 2859 sweetpotato cDNA clones were assembled into 483 clusters and 442 singletons. Comparison of sweetpotato expressed sequence tags (ESTs) to nodulation/tumorigenesis-related sequence databases (nodule-, tumor-, potato tuber- and development-related sequences) revealed that homologs of 39 sweetpotato EST sequences potentially involved in gene regulation, signal transduction and development were present in at least one of the nodulation/tumorigenesis-related sequence databases. Northern blot analyses of these 39 sequences identified 22 differentially expressed genes in early stage storage root and fibrous root. These differentially expressed genes will be potential candidates for research to elucidate the molecular processes related to sweetpotato storage root induction.
| Original language | English |
|---|---|
| Pages (from-to) | 101-105 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 536 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 2003 Feb 11 |
Bibliographical note
Funding Information:This research was supported by a Grant (PF003202-04) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean Government.
Keywords
- Comparative analysis
- Expressed sequence tag
- Ipomoea batatas
- Storage root
- Storage root induction
- Sweetpotato
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology