Identification of lignin-deficient brachypodium distachyon (L.) beauv. mutants induced by gamma radiation

Man Bo Lee, Jae Yoon Kim, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99%), 142-3 (by 13.8%) and 406-1 (by 8.13%) comparedwith the wild type. Moreover, fermentable glucosewas significantly higher in 135-2 (by 23.91%) and 142-3 (by 36.72%) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cellwall and could contribute to increases in bioethanol production using bioenergy crops.

Original languageEnglish
Pages (from-to)2159-2165
Number of pages7
JournalJournal of the Science of Food and Agriculture
Volume97
Issue number7
DOIs
Publication statusPublished - 2016 Oct 5

Keywords

  • Brachypodium distachyon
  • Enzyme hydrolysis
  • Gamma radiation
  • Lignin
  • Lignocellulosic bioethanol
  • Mutation

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Fingerprint

Dive into the research topics of 'Identification of lignin-deficient brachypodium distachyon (L.) beauv. mutants induced by gamma radiation'. Together they form a unique fingerprint.

Cite this