Identification of Newly Emerging Influenza Viruses by Detecting the Virally Infected Cells Based on Surface Enhanced Raman Spectroscopy and Principal Component Analysis

Jae Young Lim, Jung Soo Nam, Hyunku Shin, Jaena Park, Hye In Song, Minsung Kang, Kwang Il Lim, Yeonho Choi

    Research output: Contribution to journalArticlepeer-review

    58 Citations (Scopus)

    Abstract

    Rapid diagnosis and quarantine of influenza virus mutant-infected people is critical to contain the fatal viral infection spread because effective antiviral drugs are normally not available. Conventional methods, however, cannot be used for the diagnosis because these methods need predefined labels, likely also unavailable for just emerging viruses. Here, we propose label-free identification of cells infected with different influenza viruses based on surface-enhanced Raman spectroscopy (SERS) and principal component analysis (PCA). Viral envelope proteins that are displayed on the surface of cells after infection of influenza viruses were targeted for this identification. Cells that expressed the envelope proteins of A/WSN/33 H1N1 or A/California/04/2009 H1N1 influenza viruses produced distinct SERS signals. Cells that displayed combinations of the envelope proteins from these two viral variants, an indication of emergence of a new virus, also generated characteristic SERS patterns. However, the cell's own surface proteins often hindered the identification of virally infected cells by producing SERS peaks similar to viral ones. PCA of the obtained SERS patterns could effectively capture the virus-specific signal components from the jumbled SERS peaks. Our study demonstrates a potential of combination of SERS and PCA to identify newly emerging influenza viruses through sensing the cells infected with the viruses.

    Original languageEnglish
    Pages (from-to)5677-5684
    Number of pages8
    JournalAnalytical chemistry
    Volume91
    Issue number9
    DOIs
    Publication statusPublished - 2019 May 7

    Bibliographical note

    Funding Information:
    This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant HR14C0007-060018). This research was also supported by a grant from the National Research Foundation of Korea (NRF-2016M3A9B6947831). This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Animal Disease Management Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant 118094-03).

    ASJC Scopus subject areas

    • Analytical Chemistry

    Fingerprint

    Dive into the research topics of 'Identification of Newly Emerging Influenza Viruses by Detecting the Virally Infected Cells Based on Surface Enhanced Raman Spectroscopy and Principal Component Analysis'. Together they form a unique fingerprint.

    Cite this