TY - JOUR
T1 - Identification of oxidative stress and estrogenic activity induced by polarity fractions of effluent organic matter
AU - Yoo, Jisu
AU - Hur, Jin
AU - Jung, Jinho
N1 - Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2015R1D1A1A01056875 , 2018R1D1A1B07050108 ) and by a Korea University Grant .
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/8/5
Y1 - 2019/8/5
N2 - This study evaluated oxidative stress and estrogenic activity induced by hydrophobic (HP), transphilic (TP), and hydrophilic (HL)fractions of effluent organic matter (EfOM)from municipal and industrial wastewater treatment plants. Fluorescence regional integration (FRI)analysis indicated that EfOM showed higher FRI distribution in regions II (aromatic protein-like)and IV (soluble microbial products, SMPs)than Suwannee River natural organic matter (SRNOM), which was primarily in regions III (fulvic acid-like)and V (humic acid-like). The HP and HL fractions of industrial EfOMs significantly increased catalase (CAT)and glutathione S-transferase (GST)activity and lipid peroxidation compared with those of the control (p < 0.05). Estrogenic activity was the highest in industrial EfOM followed by municipal EfOM, and both were significantly higher than that of SRNOM and the control (p < 0.05). FRI distribution in region II was significantly related to CAT, GST, and estrogenic activity (r = 0.7142, 0.7786, and 0.8107, respectively)for the HL fraction of EfOM. For the HP fraction of EfOM, regions II and IV were significantly related to estrogenic activity (r = 0.7221 and 0.6707, respectively). These findings suggest that aromatic protein-like substances and SMPs in EfOM were responsible for the observed oxidative stress and estrogenic activity.
AB - This study evaluated oxidative stress and estrogenic activity induced by hydrophobic (HP), transphilic (TP), and hydrophilic (HL)fractions of effluent organic matter (EfOM)from municipal and industrial wastewater treatment plants. Fluorescence regional integration (FRI)analysis indicated that EfOM showed higher FRI distribution in regions II (aromatic protein-like)and IV (soluble microbial products, SMPs)than Suwannee River natural organic matter (SRNOM), which was primarily in regions III (fulvic acid-like)and V (humic acid-like). The HP and HL fractions of industrial EfOMs significantly increased catalase (CAT)and glutathione S-transferase (GST)activity and lipid peroxidation compared with those of the control (p < 0.05). Estrogenic activity was the highest in industrial EfOM followed by municipal EfOM, and both were significantly higher than that of SRNOM and the control (p < 0.05). FRI distribution in region II was significantly related to CAT, GST, and estrogenic activity (r = 0.7142, 0.7786, and 0.8107, respectively)for the HL fraction of EfOM. For the HP fraction of EfOM, regions II and IV were significantly related to estrogenic activity (r = 0.7221 and 0.6707, respectively). These findings suggest that aromatic protein-like substances and SMPs in EfOM were responsible for the observed oxidative stress and estrogenic activity.
KW - Effluent
KW - Estrogenic activity
KW - Organic matter
KW - Oxidative stress
KW - Polarity
UR - http://www.scopus.com/inward/record.url?scp=85065250461&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2019.05.008
DO - 10.1016/j.jhazmat.2019.05.008
M3 - Article
C2 - 31078986
AN - SCOPUS:85065250461
SN - 0304-3894
VL - 375
SP - 264
EP - 272
JO - Journal of hazardous materials
JF - Journal of hazardous materials
ER -