Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas

Eun Sil Ha; Seonyoung Choi; Kwang Ho In; Seung Hyeun Lee; Eun Joo Lee; Sang Yeub Lee; Je Hyeong Kim; Chol Shin; Jae Jeong Shim; Kyung Ho Kang; Sohee Phark; Donggeun Sul

doi:10.1016/j.clinbiochem.2012.11.014

Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas

Eun Sil Ha, Seonyoung Choi, Kwang Ho In, Seung Hyeun Lee, Eun Joo Lee, Sang Yeub Lee, Je Hyeong Kim, Chol Shin, Jae Jeong Shim, Kyung Ho Kang, Sohee Phark, Donggeun Sul

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Rationale: Among patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5. years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5. years and from those with early recurrence. Methods: Lung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36. months (early recurrence group, n=. 9) and those that were disease-free for over 5. years (disease free group, n=. 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30. ×. 40. cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins. Results: Twelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated. Conclusions: A total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.

Original language	English
Pages (from-to)	369-377
Number of pages	9
Journal	Clinical Biochemistry
Volume	46
Issue number	4-5
DOIs	https://doi.org/10.1016/j.clinbiochem.2012.11.014
Publication status	Published - 2013 Mar

Bibliographical note

Funding Information:
Lung cancer tissues were obtained from patients with pathologically confirmed stage I adenocarcinoma resected at Anam Hospital, Korea University Medical Center between December 2001 and January 2005. After surgical resection, these lung tissues were immediately frozen at − 80 °C, then donated to and stored at the Korea Lung Tissue Bank, which is supported by the Korean Science and Engineering Foundation in the Ministry of Sciences and Technology. All patients gave their consent, and the study protocol was approved by the Clinical Research Ethics Committee of Korea University Medical Center. Of the 15 subjects enrolled, 9 had recurrent lesions within 36 months of surgery (early recurrence group) and the remaining 6 remained disease-free for more than 5 years (disease free group).

Keywords

Biomarker
Lung adenocarcinoma
Prognosis
Proteomic analysis

ASJC Scopus subject areas

Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clinbiochem.2012.11.014

Cite this

@article{15e61a023d9246839a0fec633ef903b3,

title = "Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas",

abstract = "Rationale: Among patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5. years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5. years and from those with early recurrence. Methods: Lung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36. months (early recurrence group, n=. 9) and those that were disease-free for over 5. years (disease free group, n=. 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30. ×. 40. cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins. Results: Twelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated. Conclusions: A total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.",

keywords = "Biomarker, Lung adenocarcinoma, Prognosis, Proteomic analysis",

author = "Ha, {Eun Sil} and Seonyoung Choi and In, {Kwang Ho} and Lee, {Seung Hyeun} and Lee, {Eun Joo} and Lee, {Sang Yeub} and Kim, {Je Hyeong} and Chol Shin and Shim, {Jae Jeong} and Kang, {Kyung Ho} and Sohee Phark and Donggeun Sul",

note = "Funding Information: Lung cancer tissues were obtained from patients with pathologically confirmed stage I adenocarcinoma resected at Anam Hospital, Korea University Medical Center between December 2001 and January 2005. After surgical resection, these lung tissues were immediately frozen at − 80 °C, then donated to and stored at the Korea Lung Tissue Bank, which is supported by the Korean Science and Engineering Foundation in the Ministry of Sciences and Technology. All patients gave their consent, and the study protocol was approved by the Clinical Research Ethics Committee of Korea University Medical Center. Of the 15 subjects enrolled, 9 had recurrent lesions within 36 months of surgery (early recurrence group) and the remaining 6 remained disease-free for more than 5 years (disease free group). ",

year = "2013",

month = mar,

doi = "10.1016/j.clinbiochem.2012.11.014",

language = "English",

volume = "46",

pages = "369--377",

journal = "Clinical Biochemistry",

issn = "0009-9120",

publisher = "Elsevier Inc.",

number = "4-5",

}

TY - JOUR

T1 - Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas

AU - Ha, Eun Sil

AU - Choi, Seonyoung

AU - In, Kwang Ho

AU - Lee, Seung Hyeun

AU - Lee, Eun Joo

AU - Lee, Sang Yeub

AU - Kim, Je Hyeong

AU - Shin, Chol

AU - Shim, Jae Jeong

AU - Kang, Kyung Ho

AU - Phark, Sohee

AU - Sul, Donggeun

N1 - Funding Information: Lung cancer tissues were obtained from patients with pathologically confirmed stage I adenocarcinoma resected at Anam Hospital, Korea University Medical Center between December 2001 and January 2005. After surgical resection, these lung tissues were immediately frozen at − 80 °C, then donated to and stored at the Korea Lung Tissue Bank, which is supported by the Korean Science and Engineering Foundation in the Ministry of Sciences and Technology. All patients gave their consent, and the study protocol was approved by the Clinical Research Ethics Committee of Korea University Medical Center. Of the 15 subjects enrolled, 9 had recurrent lesions within 36 months of surgery (early recurrence group) and the remaining 6 remained disease-free for more than 5 years (disease free group).

PY - 2013/3

Y1 - 2013/3

N2 - Rationale: Among patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5. years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5. years and from those with early recurrence. Methods: Lung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36. months (early recurrence group, n=. 9) and those that were disease-free for over 5. years (disease free group, n=. 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30. ×. 40. cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins. Results: Twelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated. Conclusions: A total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.

AB - Rationale: Among patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5. years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5. years and from those with early recurrence. Methods: Lung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36. months (early recurrence group, n=. 9) and those that were disease-free for over 5. years (disease free group, n=. 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30. ×. 40. cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins. Results: Twelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated. Conclusions: A total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.

KW - Biomarker

KW - Lung adenocarcinoma

KW - Prognosis

KW - Proteomic analysis

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DO - 10.1016/j.clinbiochem.2012.11.014

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AN - SCOPUS:84873705551

SN - 0009-9120

VL - 46

SP - 369

EP - 377

JO - Clinical Biochemistry

JF - Clinical Biochemistry

IS - 4-5

ER -

Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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