Identification of serum biomarkers for premature ovarian failure

Da Hye Lee, Chang Zhu Pei, Jae Yun Song, Kyung Ju Lee, Bo Seong Yun, Kyu Bum Kwack, Eun Il Lee, Kwang Hyun Baek

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Premature ovarian failure (POF) is defined when a female achieves menopause before the age of 40. Although many conditions are known to be causative for POF, the most common one is idiopathic. This study was undertaken to investigate the pathogenesis of POF using proteomic tools. Two-dimensional electrophoresis (2-DE) analysis was performed to screen for proteins differentially expressed in patients with POF. Using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), we identified 11 significant proteins differentially expressed in the serum of POF patients: 5 proteins with expression increased more than two folds, 5 proteins with expression decreased more than two folds, and 1 protein expressed specifically in the serum of patients with POF. The results of the 2-DE analysis were further validated by Western blotting and ELISA analyses, which 5 reproductive system-related proteins (Ceruloplasmin, Complement C3, Fibrinogen α Fibrinogen β and SHBG) were selected. The different expression levels for these proteins were confirmed and demonstrated the possibility of using them as biomarkers to screen POF. These pre-clinical data provide plausible translational implications for targeting the pathogenesis of POF for each protein.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Issue number3
Publication statusPublished - 2019 Mar


  • Biomarker
  • Early menopause
  • Serum
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology


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