Identification of the biosynthetic gene cluster for the antibiotic polyketide L-155,175 in Streptomyces hygroscopicus

Keyphrases

• Upstream Open Reading Frame (uORF) 100%
• Antibiotics 100%
• Polyketide Synthase 100%
• Cloned Genes 100%
• Polyketides 100%
• Biosynthetic Gene Clusters 100%
• Type I Polyketide Synthase 100%
• Ketosynthase Domain 100%
• Streptomyces Hygroscopicus 100%
• Fosmid Clone 100%
• Deletion mutant 50%
• Streptomyces 50%
• Resistance Genes 50%
• Degenerate Primers 50%
• Linker 50%
• Gene Cluster 50%
• Homologous Recombination 50%
• Primer Set 50%
• Sequence Analysis 50%
• Conserved Regions 50%
• LC-MS Analysis 50%
• DNA Segment 50%
• Antifungal Compounds 50%
• Domain Organization 50%
• Genomic DNA Library 50%
• Gene Replacement 50%
• Unusual Structures 50%
• Antiparasitic Compounds 50%
• Overlapping Sequences 50%
• Apramycin 50%
• 16-membered Macrolides 50%
• Tetrahydropyran Ring 50%

Biochemistry, Genetics and Molecular Biology

• Gene Cluster 100%
• Polyketide Synthase 100%
• Polyketide 100%
• Streptomyces hygroscopicus 100%
• Anabolism 75%
• Open Reading Frame 50%
• Fosmid 50%
• DNA Library 25%
• Deletion Mutant 25%
• Protein Sequencing 25%
• Liquid Chromatography-Mass Spectrometry 25%
• Macrolide 25%
• Streptomyces 25%
• Homologous Recombination 25%
• Apramycin 25%
• Genomics 25%

Immunology and Microbiology

• Gene Cluster 100%
• Streptomyces hygroscopicus 100%
• Open Reading Frame 66%
• Fosmid 66%
• DNA Library 33%
• Deletion Mutant 33%
• Streptomyces 33%
• Sequence Analysis 33%
• Liquid Chromatography Mass Spectrometry 33%
• Homologous Recombination 33%