Abstract
mNOSTRIN is the mouse ortholog of hNOSTRIN. Unlike hNOSTRIN, which is alternatively spliced to produce two isoforms (α and β), only a single isoform of mNOSTRIN has been detected in either the nucleus or cytoplasm/membrane. Because mNOSTRIN represses its own transcription through direct binding onto its own promoter, this protein is constantly expressed in a temporally regulated pattern during differentiation of F9 embryonic carcinoma cells. In this study, we identified the specific cis-element in the mNOSTRIN regulatory region that is responsible for negative autogenous control. This element exhibits inverted dyad symmetry. Furthermore, we identified a putative bZIP motif in the middle region of mNOSTRIN, which is responsible for DNA binding, and showed that disruption of the leucine zippers abolished the DNA-binding activity of mNOSTRIN. Here, we report that a single form of mNOSTRIN functions in both the nucleus and cytoplasm/membrane. In the nucleus, mNOSTRIN acts as a transcriptional repressor by binding to the cis-element through its bZIP motif.
Original language | English |
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Pages (from-to) | 924-931 |
Number of pages | 8 |
Journal | Biochemical and biophysical research communications |
Volume | 443 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2014 Jan 17 |
Bibliographical note
Funding Information:This study was mostly aided by Grant No. ( R01-2006-000-11256-0 ) from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF) and was supported in part by professor research fund (2011) provided from Korea University.
Keywords
- Inverted-repeat
- NOSTRIN
- bZIP
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology