TY - JOUR
T1 - Idesolide inhibits the adipogenic differentiation of mesenchymal cells through the suppression of nitric oxide production
AU - Hwang, Jun Ha
AU - Moon, Sung Ah
AU - Lee, Cham Han
AU - Byun, Mi Ran
AU - Kim, A. Rum
AU - Sung, Mi Kyung
AU - Park, Hyun Jin
AU - Hwang, Eun Sook
AU - Sung, Sang Hyun
AU - Hong, Jeong Ho
N1 - Funding Information:
This work was supported by basic science research programs ( 2009-0075911 and 2011-0022926 ) and the BRL program ( 2009-0001197 ) through the National Research Foundation of Korea (NRF) . This work was also supported by Mid-Career Research Progeam ( 2009-0084879 ) and the Ewha Global Top5 Grant 2011 of Ewha Womans University.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2012/6/15
Y1 - 2012/6/15
N2 - Obesity is a major health problem worldwide and can increase the risk for several chronic diseases, including diabetes and cardiovascular disease. In this study, we screened small compounds isolated from natural products for the development of an anti-obesity drug. Among them, idesolide, a spiro compound isolated from the fruits of Idesia polycarpa Maxim, showed a significant suppression of the adipogenic differentiation in mesenchymal cells, as indicated by the decrease in fat droplets and expression of adipogenic marker genes such as aP2 and adiponectin. Idesolide inhibits the PPARλ-mediated gene transcription in a dose-dependent manner, revealed by luciferase reporter gene assay.During adipogenic differentiation, idesolide inhibits nitric oxide production through the suppression of iNOS expression, and the increased adipogenic differentiation by arginine, the substrate for NOS, is significantly inhibited by idesolide, suggesting that the inhibition of nitric oxide production plays a major role in idesolide-induced adipogenic suppression. Taken together, the results reveal that idesolide has anti-adipogenic activity and highlight its potential in the prevention and treatment of obesity.
AB - Obesity is a major health problem worldwide and can increase the risk for several chronic diseases, including diabetes and cardiovascular disease. In this study, we screened small compounds isolated from natural products for the development of an anti-obesity drug. Among them, idesolide, a spiro compound isolated from the fruits of Idesia polycarpa Maxim, showed a significant suppression of the adipogenic differentiation in mesenchymal cells, as indicated by the decrease in fat droplets and expression of adipogenic marker genes such as aP2 and adiponectin. Idesolide inhibits the PPARλ-mediated gene transcription in a dose-dependent manner, revealed by luciferase reporter gene assay.During adipogenic differentiation, idesolide inhibits nitric oxide production through the suppression of iNOS expression, and the increased adipogenic differentiation by arginine, the substrate for NOS, is significantly inhibited by idesolide, suggesting that the inhibition of nitric oxide production plays a major role in idesolide-induced adipogenic suppression. Taken together, the results reveal that idesolide has anti-adipogenic activity and highlight its potential in the prevention and treatment of obesity.
KW - Adipogenic differentiation
KW - Idesolide
KW - Nitric oxide
KW - Obesity
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U2 - 10.1016/j.ejphar.2012.04.018
DO - 10.1016/j.ejphar.2012.04.018
M3 - Article
C2 - 22546224
AN - SCOPUS:84861348597
SN - 0014-2999
VL - 685
SP - 218
EP - 223
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -
