IL-18 enhances thrombospondin-1 production in human gastric cancer via JNK pathway

Jihye Kim; Cherlhyun Kim; Tae Sung Kim; Sa Ik Bang; Young Yang; Hyunjeong Park; Daeho Cho

doi:10.1016/j.bbrc.2006.04.016

IL-18 enhances thrombospondin-1 production in human gastric cancer via JNK pathway

Jihye Kim
, Cherlhyun Kim
, Tae Sung Kim
, Sa Ik Bang
, Young Yang
, Hyunjeong Park^*
, Daeho Cho

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

IL-18 is a pleiotropic cytokine that is produced by many cancer cells. A recent report suggested that IL-18 plays a key role in regulating the immune escape of melanoma and gastric cancer cells. Thrombospondin (TSP-1) is known to inhibit angiogenesis in several cancers but some studies have reported that it stimulates angiogenesis in some cancers such as gastric cancer. IL-18 and TSP-1 are related to tumor proliferation and metastasis. This study investigated the relationship between IL-18 and TSP-1 in gastric cancer. RT-PCR and ELISA showed that after the cells had been treated with IL-18, the level of TSP-1 mRNA expression and TSP-1 protein production by IL-18 increased in both a dose- and time-dependent manner. The cells were next treated with specific inhibitors in order to determine the signal pathway involved in IL-18-enhanced TSP-1 production. IL-18-enhanced TSP-1 expression was blocked by SP600125, a c-Jun N-terminal kinase (JNK) specific inhibitor. In addition, Western blot showed that IL-18 enhanced the expression of phosphorylated JNK. Overall, these results suggest that IL-18 plays a key role in TSP-1 expression involving JNK.

Original language	English
Pages (from-to)	1284-1289
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	344
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2006.04.016
Publication status	Published - 2006 Jun 16

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) through Research Center for Women’s Diseases (R11-2005-017) at Sookmyung Women’s University, TIMRC at SNU (R13-2002-025-01002-0) and Korea Research Foundation Grant (KRF-2004-042-E00055).

Keywords

Gastric cancer
IL-18
JNK pathway
TSP-1

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2006.04.016

Cite this

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title = "IL-18 enhances thrombospondin-1 production in human gastric cancer via JNK pathway",

abstract = "IL-18 is a pleiotropic cytokine that is produced by many cancer cells. A recent report suggested that IL-18 plays a key role in regulating the immune escape of melanoma and gastric cancer cells. Thrombospondin (TSP-1) is known to inhibit angiogenesis in several cancers but some studies have reported that it stimulates angiogenesis in some cancers such as gastric cancer. IL-18 and TSP-1 are related to tumor proliferation and metastasis. This study investigated the relationship between IL-18 and TSP-1 in gastric cancer. RT-PCR and ELISA showed that after the cells had been treated with IL-18, the level of TSP-1 mRNA expression and TSP-1 protein production by IL-18 increased in both a dose- and time-dependent manner. The cells were next treated with specific inhibitors in order to determine the signal pathway involved in IL-18-enhanced TSP-1 production. IL-18-enhanced TSP-1 expression was blocked by SP600125, a c-Jun N-terminal kinase (JNK) specific inhibitor. In addition, Western blot showed that IL-18 enhanced the expression of phosphorylated JNK. Overall, these results suggest that IL-18 plays a key role in TSP-1 expression involving JNK.",

keywords = "Gastric cancer, IL-18, JNK pathway, TSP-1",

author = "Jihye Kim and Cherlhyun Kim and Kim, \{Tae Sung\} and Bang, \{Sa Ik\} and Young Yang and Hyunjeong Park and Daeho Cho",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) through Research Center for Women{\textquoteright}s Diseases (R11-2005-017) at Sookmyung Women{\textquoteright}s University, TIMRC at SNU (R13-2002-025-01002-0) and Korea Research Foundation Grant (KRF-2004-042-E00055).",

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AU - Kim, Jihye

AU - Kim, Cherlhyun

AU - Kim, Tae Sung

AU - Bang, Sa Ik

AU - Yang, Young

AU - Park, Hyunjeong

AU - Cho, Daeho

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) through Research Center for Women’s Diseases (R11-2005-017) at Sookmyung Women’s University, TIMRC at SNU (R13-2002-025-01002-0) and Korea Research Foundation Grant (KRF-2004-042-E00055).

PY - 2006/6/16

Y1 - 2006/6/16

N2 - IL-18 is a pleiotropic cytokine that is produced by many cancer cells. A recent report suggested that IL-18 plays a key role in regulating the immune escape of melanoma and gastric cancer cells. Thrombospondin (TSP-1) is known to inhibit angiogenesis in several cancers but some studies have reported that it stimulates angiogenesis in some cancers such as gastric cancer. IL-18 and TSP-1 are related to tumor proliferation and metastasis. This study investigated the relationship between IL-18 and TSP-1 in gastric cancer. RT-PCR and ELISA showed that after the cells had been treated with IL-18, the level of TSP-1 mRNA expression and TSP-1 protein production by IL-18 increased in both a dose- and time-dependent manner. The cells were next treated with specific inhibitors in order to determine the signal pathway involved in IL-18-enhanced TSP-1 production. IL-18-enhanced TSP-1 expression was blocked by SP600125, a c-Jun N-terminal kinase (JNK) specific inhibitor. In addition, Western blot showed that IL-18 enhanced the expression of phosphorylated JNK. Overall, these results suggest that IL-18 plays a key role in TSP-1 expression involving JNK.

AB - IL-18 is a pleiotropic cytokine that is produced by many cancer cells. A recent report suggested that IL-18 plays a key role in regulating the immune escape of melanoma and gastric cancer cells. Thrombospondin (TSP-1) is known to inhibit angiogenesis in several cancers but some studies have reported that it stimulates angiogenesis in some cancers such as gastric cancer. IL-18 and TSP-1 are related to tumor proliferation and metastasis. This study investigated the relationship between IL-18 and TSP-1 in gastric cancer. RT-PCR and ELISA showed that after the cells had been treated with IL-18, the level of TSP-1 mRNA expression and TSP-1 protein production by IL-18 increased in both a dose- and time-dependent manner. The cells were next treated with specific inhibitors in order to determine the signal pathway involved in IL-18-enhanced TSP-1 production. IL-18-enhanced TSP-1 expression was blocked by SP600125, a c-Jun N-terminal kinase (JNK) specific inhibitor. In addition, Western blot showed that IL-18 enhanced the expression of phosphorylated JNK. Overall, these results suggest that IL-18 plays a key role in TSP-1 expression involving JNK.

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KW - IL-18

KW - JNK pathway

KW - TSP-1

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ER -

IL-18 enhances thrombospondin-1 production in human gastric cancer via JNK pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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