IL-32γ induces chemotaxis of activated T cells via dendritic cell-derived CCL5

Mi Hye Son, Mi Young Jung, Seulah Choi, Daeho Cho, Tae Sung Kim

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Interleukin (IL)-32 has been associated with a variety of inflammatory diseases including rheumatoid arthritis, vasculitis and Crohn's disease. We have previously reported that IL-32γ, the IL-32 isoform with the highest biological activity, could act as an immune modulator through regulation of dendritic cell (DC) functions in immune responses. Cell locomotion is crucial for induction of an effective immune response. In this study, we investigated the effect and underlying mechanisms of IL-32γ on recruitment of T cells. IL-32γ upregulated the expression of several chemokines including CCL2, CCL4, and CCL5 in the DCs. In particular, IL-32γ significantly increased CCL5 expression in a dose-dependent manner. Treatment with JNK and NF-κB inhibitors suppressed IL-32γ-induced CCL5 expression in DCs, indicating that IL-32γ induced CCL5 production through the JNK and NF-κB pathways. Furthermore, supernatants from IL-32γ-treated DCs showed chemotactic activities controlling migration of activated CD4+ and CD8+ T cells, and these activities were suppressed by addition of neutralizing anti-CCL5 antibody. These results show that IL-32γ effectively promotes migration of activated T cells via CCL5 production in DCs. The chemotactic potential of IL-32γ may explain the pro-inflammatory effects of IL-32 and the pathologic role of IL-32 in immune disorders such as rheumatoid arthritis.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalBiochemical and biophysical research communications
Issue number1
Publication statusPublished - 2014 Jul 18

Bibliographical note

Funding Information:
We thank Hye-Jin Hong and Hui Xuan Lim for many helpful insights and technical assistances. This work was supported by a grant of the R&D Convergence Center Support Program of the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (to T.S.K.).


  • Activated T cells
  • CCL5
  • Chemotaxis
  • Dendritic cells
  • IL-32

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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