IL32γ activates natural killer receptor-expressing innate immune cells to produce IFNγ via dendritic cell-derived IL12

Sung Won Lee, Hyun Jung Park, Kwang Soo Lee, Se Ho Park, Soohyun Kim, Sung Ho Jeon, Seokmann Hong

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    The inflammatory cytokine IL32γ acts on dendritic cells (DCs) to produce IL12 and IL6, which are involved in the differentiation of Th1 and Th17 cells. Natural killer (NK) and NKT cells play important roles in IL12-mediated adaptive immune responses, such as antitumor immunity. Herein we demonstrate the effect of IL32γ on the activation of NK and NKT cells. Upon IL32γ stimulation, splenic NK and NKT cells could be activated, and this activation was dependent on both IL12 and DCs, which was confirmed by using IL12p35 knockout and CD11c-diphtheria toxin receptor transgenic mouse models. Furthermore, IL32γ could induce the production of proinflammatory cytokines by NKDCs, a subset of DCs expressing NK cell markers, known to enhance NKT cell function. Unlike conventional DCs, NKDCs produced IFNγ and TNFα rather than IL12 upon stimulation with IL32γ. Taken together, IL32γ will be useful as an adjuvant to boost the cytotoxicities of NK and NKT cells that play critical roles in antitumor immunity.

    Original languageEnglish
    Pages (from-to)86-94
    Number of pages9
    JournalBiochemical and biophysical research communications
    Volume461
    Issue number1
    DOIs
    Publication statusPublished - 2015 May 22

    Bibliographical note

    Publisher Copyright:
    © 2015 Elsevier Inc. All rights reserved.

    Keywords

    • IFNγ
    • IL12
    • IL32γ
    • NK
    • NKDC
    • NKT

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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