TY - JOUR
T1 - Immobilization of glucose oxidase into polyaniline nanofiber matrix for biofuel cell applications
AU - Kim, Hyeongseok
AU - Lee, Inseon
AU - Kwon, Yongchai
AU - Kim, Byoung Chan
AU - Ha, Su
AU - Lee, Jung heon
AU - Kim, Jungbae
N1 - Funding Information:
This work was supported by grants from the National Research Foundation (NRF) funded by the Korean Ministry of Education, Science & Technology (MEST) ( 2009-0082314 , 2009-0075638 , 2009-0084771 , and K20902001448-10E0100-03010 ).
PY - 2011/5/15
Y1 - 2011/5/15
N2 - Glucose oxidase (GOx) was immobilized into the porous matrix of polyaniline nanofibers in a three-step process, consisting of enzyme adsorption, precipitation, and crosslinking (EAPC). EAPC was highly active and stable when compared to the control samples of enzyme adsorption (EA) and enzyme adsorption and crosslinking (EAC) with no step of enzyme precipitation. The GOx activity of EAPC was 9.6 and 4.2 times higher than those of EA and EAC, respectively. Under rigorous shaking at room temperature for 56days, the relative activities of EA, EAC and EAPC, defined as the percentage of residual activity to the initial activity, were 22%, 19% and 91%, respectively. When incubated at 50°C under shaking for 4h, EAPC showed a negligible decrease of GOx activity while the relative activities of EA and EAC were 45% and 48%, respectively. To demonstrate the feasible application of EAPC in biofuel cells, the enzyme anodes were prepared and used for home-built air-breathing biofuel cells. The maximum power densities of biofuel cells with EA and EAPC anodes were 57 and 292μW/cm2, respectively. After thermal treatment at 60°C for 4h, the maximum power density of EA and EAPC anodes were 32 and 315μW/cm2, representing 56% and 108% of initially obtained maximum power densities, respectively. Because the lower power densities and short lifetime of biofuel cells are serious problems against their practical applications, the present results with EAPC anode has opened up a new potential for the realization of practical biofuel cell applications.
AB - Glucose oxidase (GOx) was immobilized into the porous matrix of polyaniline nanofibers in a three-step process, consisting of enzyme adsorption, precipitation, and crosslinking (EAPC). EAPC was highly active and stable when compared to the control samples of enzyme adsorption (EA) and enzyme adsorption and crosslinking (EAC) with no step of enzyme precipitation. The GOx activity of EAPC was 9.6 and 4.2 times higher than those of EA and EAC, respectively. Under rigorous shaking at room temperature for 56days, the relative activities of EA, EAC and EAPC, defined as the percentage of residual activity to the initial activity, were 22%, 19% and 91%, respectively. When incubated at 50°C under shaking for 4h, EAPC showed a negligible decrease of GOx activity while the relative activities of EA and EAC were 45% and 48%, respectively. To demonstrate the feasible application of EAPC in biofuel cells, the enzyme anodes were prepared and used for home-built air-breathing biofuel cells. The maximum power densities of biofuel cells with EA and EAPC anodes were 57 and 292μW/cm2, respectively. After thermal treatment at 60°C for 4h, the maximum power density of EA and EAPC anodes were 32 and 315μW/cm2, representing 56% and 108% of initially obtained maximum power densities, respectively. Because the lower power densities and short lifetime of biofuel cells are serious problems against their practical applications, the present results with EAPC anode has opened up a new potential for the realization of practical biofuel cell applications.
KW - Enzyme precipitation
KW - Enzyme stabilization
KW - Enzyme-based biofuel cells
KW - Glucose oxidase
KW - Polyaniline nanofibers
UR - http://www.scopus.com/inward/record.url?scp=79955480853&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.03.008
DO - 10.1016/j.bios.2011.03.008
M3 - Article
C2 - 21470844
AN - SCOPUS:79955480853
SN - 0956-5663
VL - 26
SP - 3908
EP - 3913
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 9
ER -