Immobilization of lipase on single walled carbon nanotubes in ionic liquid

Han Ki Lee; Jae Kwan Lee; Mahn Joo Kim; Cheol Jin Lee

doi:10.5012/bkcs.2010.31.03.650

Immobilization of lipase on single walled carbon nanotubes in ionic liquid

Han Ki Lee, Jae Kwan Lee, Mahn Joo Kim, Cheol Jin Lee

School of Electrical Engineering

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF₄). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

Original language	English
Pages (from-to)	650-652
Number of pages	3
Journal	Bulletin of the Korean Chemical Society
Volume	31
Issue number	3
DOIs	https://doi.org/10.5012/bkcs.2010.31.03.650
Publication status	Published - 2010 Mar

Keywords

Biocatalysis
Immobilization
Ionic liquid
Lipase
Single walled carbon nanotube

ASJC Scopus subject areas

Chemistry(all)

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10.5012/bkcs.2010.31.03.650

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title = "Immobilization of lipase on single walled carbon nanotubes in ionic liquid",

abstract = "A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.",

keywords = "Biocatalysis, Immobilization, Ionic liquid, Lipase, Single walled carbon nanotube",

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AU - Lee, Han Ki

AU - Lee, Jae Kwan

AU - Kim, Mahn Joo

AU - Lee, Cheol Jin

PY - 2010/3

Y1 - 2010/3

N2 - A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

AB - A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-BF4). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.

KW - Biocatalysis

KW - Immobilization

KW - Ionic liquid

KW - Lipase

KW - Single walled carbon nanotube

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JO - Bulletin of the Korean Chemical Society

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Immobilization of lipase on single walled carbon nanotubes in ionic liquid

Abstract

Keywords

ASJC Scopus subject areas

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