Abstract
Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s -1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.
Original language | English |
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Pages (from-to) | 1307-1313 |
Number of pages | 7 |
Journal | Biotechnology letters |
Volume | 34 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2012 Jul |
Externally published | Yes |
Bibliographical note
Copyright:Copyright 2012 Elsevier B.V., All rights reserved.
Keywords
- Immobilization
- Response surface methodology
- SiO
- Xylanase
- Xylooligosaccharide
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology