Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k cat value (4,261 s -1) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.
Copyright 2012 Elsevier B.V., All rights reserved.
- Response surface methodology
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology