Immobilized Candida antarctica lipase B as an sn-1,3 regiospecific biocatalyst for the interesterification of triacylglycerols with fatty acid ethyl esters

Jihyun Hwang, Jiae Aum, Seong Jae Lee, Jeong Min Mun, Seung Wook Kim, Min Yu Chung, In Hwan Kim, Byung Hee Kim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Candida antarctica lipase B (CALB) is regarded as non-regiospecific. This study aimed to investigate the regiospecificity of CALB in the solvent-free interesterification of high-oleic sunflower oil with stearic acid ethyl ester for 1,3-distearoyl-2-oleoylglycerol (SOS)-rich fat preparation using a packed bed reactor. The content ratio of 1,2-distearoyl-3-oleoylglycerol (SSO) to SOS (denoted by SSO/SOS content) obtained using Lipozyme 435 (a commercially immobilized CALB; 0–4.1%), at residence times (1–32 min) was similar to that obtained using Lipozyme RM IM (0–3.0%), but lower than that obtained using Lipozyme TL IM (6.0–39.4%). When immobilized on Lewatit VP OC 1600, Lipozyme CALB had an SSO/SOS content of 0–10.4%, which was greater than that of Palatase 20,000 L (0–1.1%) but was lower than that of Lipozyme TL 100 L (8.8–97.7%). Our findings suggest that immobilized CALB shows distinct sn-1,3 regiospecificity in the interesterification of triacylglycerol with fatty acid ethyl esters.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalFood Science and Biotechnology
Volume33
Issue number1
DOIs
Publication statusPublished - 2024 Jan

Bibliographical note

Publisher Copyright:
© 2023, The Korean Society of Food Science and Technology.

Keywords

  • Candida antarctica Lipase B
  • Immobilization
  • Interesterification
  • Lipozyme 435
  • Sn-1,3 specificity

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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