Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation

Tae Young Seo; Ki Won Eum; Sung Ok Han; Seung Wook Kim; Ji Hyeon Kim; Kwang Ho Song; Jaehoon Choe

doi:10.1016/j.procbio.2012.03.019

Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation

Tae Young Seo, Ki Won Eum, Sung Ok Han, Seung Wook Kim, Ji Hyeon Kim, Kwang Ho Song, Jaehoon Choe

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis.

Original language	English
Pages (from-to)	1011-1015
Number of pages	5
Journal	Process Biochemistry
Volume	47
Issue number	6
DOIs	https://doi.org/10.1016/j.procbio.2012.03.019
Publication status	Published - 2012 Jun

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant (no. 2010-0027563 ) funded by the Korea government (MEST) and the Human Resources Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant (no. 20114010203070 ) funded by the Korea government Ministry of Knowledge Economy.

Keywords

Catabolite repression
Continuous fermentation
Immobilized cell microchannel bioreactor
Pichia stipitis
Saccharomyces cerevisiae

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2012.03.019

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title = "Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation",

abstract = "An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis.",

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note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant (no. 2010-0027563 ) funded by the Korea government (MEST) and the Human Resources Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant (no. 20114010203070 ) funded by the Korea government Ministry of Knowledge Economy.",

year = "2012",

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doi = "10.1016/j.procbio.2012.03.019",

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AU - Eum, Ki Won

AU - Han, Sung Ok

AU - Kim, Seung Wook

AU - Kim, Ji Hyeon

AU - Song, Kwang Ho

AU - Choe, Jaehoon

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant (no. 2010-0027563 ) funded by the Korea government (MEST) and the Human Resources Development Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant (no. 20114010203070 ) funded by the Korea government Ministry of Knowledge Economy.

PY - 2012/6

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N2 - An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis.

AB - An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis.

KW - Catabolite repression

KW - Continuous fermentation

KW - Immobilized cell microchannel bioreactor

KW - Pichia stipitis

KW - Saccharomyces cerevisiae

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Immobilized cell microchannel bioreactor for evaluating fermentation characteristics of mixed substrate consumption and product formation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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