Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost

Woo Joo Kim; Christine C. Roberts; Joon Young Song; Jin Gu Yoon; Hye Seong; Hak Jun Hyun; Hyojin Lee; Areum Gil; Yeeun Oh; Ji Eun Park; Ji Eun Lee; Bohyun Jeon; Deborah Kane; Susan Spruill; Sagar B. Kudchodkar; Kar Muthumani; Young K. Park; Ijoo Kwon; Joel N. Maslow

doi:10.1016/j.vaccine.2023.06.013

Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost

Woo Joo Kim
, Christine C. Roberts
, Joon Young Song
, Jin Gu Yoon
, Hye Seong
, Hak Jun Hyun
, Hyojin Lee
, Areum Gil
, Yeeun Oh
, Ji Eun Park
, Ji Eun Lee
, Bohyun Jeon
, Deborah Kane
, Susan Spruill
, Sagar B. Kudchodkar
, Kar Muthumani
, Young K. Park
, Ijoo Kwon
, Joel N. Maslow^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Heterologous boost regimens are being increasingly considered against SARS-CoV-2. We report results for the 32 of 45 participants in the Phase 1 CoV2-001 clinical trial (Kim et al., Int J Iinfect Dis 2023, 128:112–120) who elected to receive an EUA-approved SARS-CoV-2 mRNA vaccine 6 to 8 months following a two-dose primary vaccination with the GLS-5310 bi-cistronic DNA vaccine given intradermally and followed by application of suction using the GeneDerm device. Receipt of EUA-approved mRNA vaccines after GLS-5310 vaccination was well-tolerated, with no reported adverse events. Immune responses were enhanced such that binding antibody titers, neutralizing antibody titers, and T-cell responses increased 1,187-fold, 110-fold, and 2.9-fold, respectively. This paper is the first description of the immune responses following heterologous vaccination with a DNA primary series and mRNA boost.

Original language	English
Pages (from-to)	4206-4211
Number of pages	6
Journal	Vaccine
Volume	41
Issue number	29
DOIs	https://doi.org/10.1016/j.vaccine.2023.06.013
Publication status	Published - 2023 Jun 29

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2023.06.013

Cite this

Kim, W. J., Roberts, C. C., Song, J. Y., Yoon, J. G., Seong, H., Hyun, H. J., Lee, H., Gil, A., Oh, Y., Park, J. E., Lee, J. E., Jeon, B., Kane, D., Spruill, S., Kudchodkar, S. B., Muthumani, K., Park, Y. K., Kwon, I., & Maslow, J. N. (2023). Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost. Vaccine, 41(29), 4206-4211. https://doi.org/10.1016/j.vaccine.2023.06.013

Kim, WJ, Roberts, CC, Song, JY, Yoon, JG, Seong, H, Hyun, HJ, Lee, H, Gil, A, Oh, Y, Park, JE, Lee, JE, Jeon, B, Kane, D, Spruill, S, Kudchodkar, SB, Muthumani, K, Park, YK, Kwon, I & Maslow, JN 2023, 'Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost', Vaccine, vol. 41, no. 29, pp. 4206-4211. https://doi.org/10.1016/j.vaccine.2023.06.013

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title = "Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost",

abstract = "Heterologous boost regimens are being increasingly considered against SARS-CoV-2. We report results for the 32 of 45 participants in the Phase 1 CoV2-001 clinical trial (Kim et al., Int J Iinfect Dis 2023, 128:112–120) who elected to receive an EUA-approved SARS-CoV-2 mRNA vaccine 6 to 8 months following a two-dose primary vaccination with the GLS-5310 bi-cistronic DNA vaccine given intradermally and followed by application of suction using the GeneDerm device. Receipt of EUA-approved mRNA vaccines after GLS-5310 vaccination was well-tolerated, with no reported adverse events. Immune responses were enhanced such that binding antibody titers, neutralizing antibody titers, and T-cell responses increased 1,187-fold, 110-fold, and 2.9-fold, respectively. This paper is the first description of the immune responses following heterologous vaccination with a DNA primary series and mRNA boost.",

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doi = "10.1016/j.vaccine.2023.06.013",

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AU - Seong, Hye

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AU - Gil, Areum

AU - Oh, Yeeun

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AU - Lee, Ji Eun

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AU - Kane, Deborah

AU - Spruill, Susan

AU - Kudchodkar, Sagar B.

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AU - Park, Young K.

AU - Kwon, Ijoo

AU - Maslow, Joel N.

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ER -

Immune response enhancement with GLS-5310 DNA primary vaccine against SARS-CoV-2 followed by administration of an mRNA vaccine heterologous boost

Abstract

Bibliographical note

UN SDGs

ASJC Scopus subject areas

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