Immunoregulation of macrophages by dynamic ligand presentation via ligand–cation coordination

Heemin Kang, Boguang Yang, Kunyu Zhang, Qi Pan, Weihao Yuan, Gang Li, Liming Bian

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Macrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg 2+ -functional Mg 2+ -BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg 2+ -BP coordination, thus producing (Mg 2+ -BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg 2+ chelation facilitates the dissolution of Mg 2+ -BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg 2+ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could damage cells. The swift formation of RGD ligand- and Mg 2+ -bifunctional RGD-Mg 2+ -BP NP that yields (RGD-Mg 2+ -BP)-Au dimer (RGDBNP) further stimulates the adhesion and pro-regenerative M2-type polarization of macrophages, both in vitro and in vivo, including rho-associated protein kinase. This swift and non-toxic dimer formation can include diverse bio-functional moieties to regulate host responses to implants.

Original languageEnglish
Article number1696
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Bibliographical note

Funding Information:
Project 31570979 is supported by the National Natural Science Foundation of China. This work is supported by a General Research Fund grant from the Research Grants Council of Hong Kong (project no. 14220716 & 14205817); the Health and Medical Research Fund, the Food and Health Bureau, the Government of the Hong Kong Special Administrative Region (reference no.: 04152836, 03140056); the Chow Yuk Ho Technology Centre for Innovative Medicine, The Chinese University of Hong Kong. This work is supported by Shenzhen Science and Technology Innovation Commission Project JCYJ20170307165611557. This work was supported by a Korea University Grant. The work was partially supported by grants from Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. 14120118, 14160917, 9054014 N_CityU102/15, T13-402/17-N); National Natural Science Foundation of China (81772404, 81430049 and 81772322); Hong Kong Innovation Technology Commission Funds (ITS/UIM-305). This study was also supported in part by SMART program, Lui Che Woo Institute of Innovative Medicine.

Publisher Copyright:
© 2019, The Author(s).

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy

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