Abstract
Korean fresh ginseng was cultured with Hericium erinaceum mycelia (HE) in solid-state culture (SSC) to enhance the immunomodulation activity. Hot-water extracts (FG-HE-HW) of H. erinaceum-fermented ginseng (FG-HE) fractionated into a crude polysaccharide (FG-HE-CP) showed higher mitogenic, macrophage stimulation, and intestinal immune system modulation activities (1.52, 1.63, and 1.35x relative to a saline control at 100 μg/mL, respectively) than the crude polysaccharide from non-fermented ginseng (NG-CP; 1.35, 1.50, and 1.18x) and H. erinaceum mycelia alone (HE-CP; 1.26, 1.46, and 1.27x). After FG-HE-CP was further fractionated on a DEAE-Sepharose CL-6B column, the polysaccharide fraction (FG-HE-CP-III) exhibited larger activity increases than any subfraction from NG-CP or HE-CP. FG-HE-CP-III mainly consisted of Ara, Gal, Glc, and GalA (molar ratios of 0.45:1.00: 0.51:0.73). NaIO4 oxidation decreased the activities of FG-HE-CP-III (51.0-76.9%). The polysaccharide from H. erinaceum-fermented ginseng using SSC plays an important role for enhancement of immunomodulation activities of fresh ginseng.
Original language | English |
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Pages (from-to) | 311-318 |
Number of pages | 8 |
Journal | Food Science and Biotechnology |
Volume | 25 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2016 Feb 1 |
Bibliographical note
Funding Information:This research was supported by a grant from the Academic Research Program of Korea National University of Transportation in 2014.
Publisher Copyright:
© 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
Keywords
- Hericium erinaceum
- fermented ginseng
- immunostimulation
- polysaccharide
- solid-state culture
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology