Immunostimulatory Polysaccharide Fractionated from a Liquid Culture by Phellinus linteus Mycelium

Hyun Young Shin, Hoon Kim, Eun Jin Jeong, Hyun Gyeong Kim, Jung Cheul Shin, Sang Young Choi, Hyung Joo Suh, Sung Kwon Moon, Kwang Won Yu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


A liquid culture was fermented using Phellinus linteus mycelium, and 5 fractions were isolated to evaluate the possibility of using them as postbiotic ingredients. The fractions isolated were: Hot-water extract (PL-HW) from whole liquid culture, crude polysaccharide (PL-CP) from PL-HW by ethanol (EtOH) precipitation, hot-water extract (PM-HW) from isolated mycelia, crude polysaccharide (PM-CP) from PM-HW, and exopolysaccharide (EPS) from culture supernatant by EtOH precipitation. Innate immunostimulating activity using the RAW 264.7 cell line showed that EPS induced the most effective production of tumor necrosis factor-alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), and nitric oxide. In addition, adaptive immunostimulating activity using splenocytes isolated from C3H/HeN mice revealed that PL-HW, PM-HW, and EPS induced prominent splenocyte proliferation. Specifically, EPS stimulated the production of Th1-activated cytokines such as TNF-α and interferon-gamma (IFN-γ) in splenocytes, suggesting its effectiveness in cell-mediated adaptive immune activation. General chemical analysis showed that the immunostimulatory EPS was composed of neutral sugar (90.2%), uronic acid (6.1%), protein (1.2%), and polyphenols (2.5%). Component sugar analysis indicated that EPS was composed of a high proportion of glucose (94.9%), and small amounts of mannose (3.8%), galactose (0.9%), and glucuronic acid (0.4%). In conclusion, it was confirmed that glucan-type exopolysaccharide fractionated from the culture supernatant of the P. linteus mycelium can promote both innate and adaptive immune cell stimulation.

Original languageEnglish
Pages (from-to)19-27
Number of pages9
JournalJournal of the Korean Society of Food Science and Nutrition
Issue number1
Publication statusPublished - 2022 Jan
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 The Korean Society of Food Science and Nutrition.


  • Exopolysaccharide
  • Innate immunostimulating activity
  • Liquid culture
  • Phellinus linteus mycelium
  • Splenocyte

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


Dive into the research topics of 'Immunostimulatory Polysaccharide Fractionated from a Liquid Culture by Phellinus linteus Mycelium'. Together they form a unique fingerprint.

Cite this