TY - JOUR
T1 - Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes
AU - Kim, Duck Gyun
AU - Yoo, Seok Woo
AU - Kim, Minsun
AU - Ko, Ja Kyong
AU - Um, Youngsoon
AU - Oh, Min Kyu
N1 - Funding Information:
This research was supported by the National Research Foundation (NRF) of South Korea funded by the Korean Government (2012M1A2A2026560 and 2018M3A9F3079643).
Funding Information:
This research was supported by the National Research Foundation (NRF) of South Korea funded by the Korean Government ( 2012M1A2A2026560 and 2018M3A9F3079643 ).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/8
Y1 - 2020/8
N2 - We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed-batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass.
AB - We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed-batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass.
KW - 2,3-butanediol
KW - Enterobacter aerogenes
KW - Lignocellulosic biomass
KW - Metabolic engineering
UR - http://www.scopus.com/inward/record.url?scp=85083460806&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2020.123386
DO - 10.1016/j.biortech.2020.123386
M3 - Article
C2 - 32330805
AN - SCOPUS:85083460806
SN - 0960-8524
VL - 309
JO - Bioresource technology
JF - Bioresource technology
M1 - 123386
ER -