We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed-batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation (NRF) of South Korea funded by the Korean Government (2012M1A2A2026560 and 2018M3A9F3079643).
This research was supported by the National Research Foundation (NRF) of South Korea funded by the Korean Government ( 2012M1A2A2026560 and 2018M3A9F3079643 ).
© 2020 Elsevier Ltd
- Enterobacter aerogenes
- Lignocellulosic biomass
- Metabolic engineering
ASJC Scopus subject areas
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal