Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin

Sung Bin Lim; Kyung Woo Kim; Chang Woo Lee; Hyo Sun Kim; Chang Soo Lee; Min Kyu Oh

doi:10.1007/s12257-008-0055-y

Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin

Sung Bin Lim, Kyung Woo Kim, Chang Woo Lee, Hyo Sun Kim, Chang Soo Lee, Min Kyu Oh

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

Original language	English
Pages (from-to)	683-689
Number of pages	7
Journal	Biotechnology and Bioprocess Engineering
Volume	13
Issue number	6
DOIs	https://doi.org/10.1007/s12257-008-0055-y
Publication status	Published - 2008

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the Korea Foundation for International Cooperation of Science & Technology (KICOS) grant number K20601000002-07E0100 -00230, the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603) and Seoul Research and Business Development Program 10920.

Keywords

Avidin-biotin conjugation
DNAchip
Fluorescence
PAMAM dendrimer

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-008-0055-y

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title = "Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin",

abstract = "Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.",

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author = "Lim, {Sung Bin} and Kim, {Kyung Woo} and Lee, {Chang Woo} and Kim, {Hyo Sun} and Lee, {Chang Soo} and Oh, {Min Kyu}",

note = "Funding Information: Acknowledgements This work was supported by the Korea Foundation for International Cooperation of Science & Technology (KICOS) grant number K20601000002-07E0100 -00230, the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603) and Seoul Research and Business Development Program 10920.",

year = "2008",

doi = "10.1007/s12257-008-0055-y",

language = "English",

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AU - Lim, Sung Bin

AU - Kim, Kyung Woo

AU - Lee, Chang Woo

AU - Kim, Hyo Sun

AU - Lee, Chang Soo

AU - Oh, Min Kyu

N1 - Funding Information: Acknowledgements This work was supported by the Korea Foundation for International Cooperation of Science & Technology (KICOS) grant number K20601000002-07E0100 -00230, the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603) and Seoul Research and Business Development Program 10920.

PY - 2008

Y1 - 2008

N2 - Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

AB - Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

KW - Avidin-biotin conjugation

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KW - Fluorescence

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ER -

Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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