Improved DNA chip with poly(amidoamine) dendrimer peripherally modified with biotin and avidin

Sung Bin Lim, Kyung Woo Kim, Chang Woo Lee, Hyo Sun Kim, Chang Soo Lee, Min Kyu Oh

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Important properties for a biosensor are the sensitive detection of target DNA at low concentration, the specific and accurate distinction of the target and other DNA having a similar sequence, and measurement capability over a wide range of target concentrations. To these ends, generation 3 polyamidoamine (PAMAM) dendrimer was used to improve DNA chip properties. PAMAM dendrimer surface amine moieties were modified to biotin and immobilized on glass slides using biotin-avidin conjugation. The surface morphologies of the avidin-biotin-dendrimer complexes were observed using atomic force microscopy and scanning electron microscopy. Detection sensitivity for fluorescence-labeled target DNA increased approximately 4-fold by the dendrimer coating. Dendrimer coating also markedly improved the dynamic range and detection of single nucleotide polymorphisms. Dendrimer complex morphology had little effect on the sensitivity.

    Original languageEnglish
    Pages (from-to)683-689
    Number of pages7
    JournalBiotechnology and Bioprocess Engineering
    Volume13
    Issue number6
    DOIs
    Publication statusPublished - 2008

    Bibliographical note

    Funding Information:
    Acknowledgements This work was supported by the Korea Foundation for International Cooperation of Science & Technology (KICOS) grant number K20601000002-07E0100 -00230, the Korea Research Foundation Grant funded by the Korean Government (KRF-2006-005-J03603) and Seoul Research and Business Development Program 10920.

    Keywords

    • Avidin-biotin conjugation
    • DNAchip
    • Fluorescence
    • PAMAM dendrimer

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Biomedical Engineering

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