Improved production of 1,3-propanediol from biodiesel-derived crude glycerol by Klebsiella pneumoniae in fed-batch fermentation

Xiaoguang Yang, Han Suk Choi, Ju Hun Lee, Soo Kwon Lee, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


In this study, crude glycerol was demonstrated as feedstock to use in the fed-batch fermentation of 1,3-propanediol production by Klebsiella pneumoniae ATCC 8724. To increase the yield and productivity of 1,3-propanediol, several factors for fed-batch fermentation were investigated, which included: initial substrate conditions, feeding strategy and fermentation conditions. Both performances of 1,3-propandiol and by-products were observed and studied for improved fed-batch fermentation. Increasing of initial working volume, continuous feeding, and culture pH switch strategy showed positive performance for this fed-batch process. The maximum 1,3-propandiol concentration, yield, and productivity were achieved 62.72 g/l, 0.73 mol/mol and 1.74 g/l/h, respectively. With the ignorable inhibitory effect, a large quantity of crude glycerol from industrial biodiesel process would be an attractive feedstock for value-added chemical, 1,3-propandiol, manufacture. The improvement of fed-batch fermentation could also be achieved by strategy optimization in further applications.

Original languageEnglish
Pages (from-to)25-36
Number of pages12
JournalChemical Engineering Journal
Publication statusPublished - 2018 Oct 1

Bibliographical note

Funding Information:
This work was supported by the Industrial Strategic Technology Development Program ( 10051513 ), funded by the Ministry of Trade, Industry & Energy (MI, Korea). This study was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( NRF-2016R1D1A1A09918327 ).

Publisher Copyright:
© 2018


  • 1,3-propanediol
  • By-products
  • Crude glycerol
  • Fed-batch strategy
  • Klebsiella pneumoniae

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Industrial and Manufacturing Engineering


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