Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes

Hwi Min Jung; Ju Yeon Lee; Jung Hyun Lee; Min Kyu Oh

doi:10.1016/j.biortech.2018.03.081

Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes

Hwi Min Jung, Ju Yeon Lee, Jung Hyun Lee, Min Kyu Oh

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m⁻² h⁻¹ isobutanol with 6–25 g L⁻¹ ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.

Original language	English
Pages (from-to)	373-380
Number of pages	8
Journal	Bioresource technology
Volume	259
DOIs	https://doi.org/10.1016/j.biortech.2018.03.081
Publication status	Published - 2018 Jul

Bibliographical note

Funding Information:
This study was supported by a grant from the National Research Foundation funded by the Korean Government (2012M1A2A2026560 and 2017R1A2B4008758).

Funding Information:
This study was supported by a grant from the National Research Foundation funded by the Korean Government ( 2012M1A2A2026560 and 2017R1A2B4008758 ).

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

Enterobacter aerogenes
Isobutanol
Lignocellulosic biomass
Pervaporation
Sugarcane bagasse hydrolysate

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2018.03.081

Cite this

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title = "Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes",

abstract = "A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m−2 h−1 isobutanol with 6–25 g L−1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.",

keywords = "Enterobacter aerogenes, Isobutanol, Lignocellulosic biomass, Pervaporation, Sugarcane bagasse hydrolysate",

author = "Jung, {Hwi Min} and Lee, {Ju Yeon} and Lee, {Jung Hyun} and Oh, {Min Kyu}",

note = "Funding Information: This study was supported by a grant from the National Research Foundation funded by the Korean Government (2012M1A2A2026560 and 2017R1A2B4008758). Funding Information: This study was supported by a grant from the National Research Foundation funded by the Korean Government ( 2012M1A2A2026560 and 2017R1A2B4008758 ). Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

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N1 - Funding Information: This study was supported by a grant from the National Research Foundation funded by the Korean Government (2012M1A2A2026560 and 2017R1A2B4008758). Funding Information: This study was supported by a grant from the National Research Foundation funded by the Korean Government ( 2012M1A2A2026560 and 2017R1A2B4008758 ). Publisher Copyright: © 2018 Elsevier Ltd

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N2 - A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m−2 h−1 isobutanol with 6–25 g L−1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.

AB - A process of isobutanol production from sugarcane bagasse hydrolysates in Enterobacter aerogenes was developed here with a pervaporation-integrated procedure. Isobutanol pathway was overexpressed in a mutant strain with eliminated byproduct-forming enzymes (LdhA, BudA, and PflB). A glucose-and-xylose–coconsuming ptsG mutant was constructed for effective utilization of lignocellulosic biomass. Toxic effects of isobutanol were alleviated by in situ recovery via a pervaporation procedure. Compared to single-batch fermentation, cell growth and isobutanol titer were improved by 60% and 100%, respectively, in the pervaporation-integrated fermentation process. A lab-made cross-linked polydimethylsiloxane membrane was cast on polyvinylidene fluoride and used in the pervaporation process. The membrane-penetrating condensate contained 55–226 g m−2 h−1 isobutanol with 6–25 g L−1 ethanol after separation. This study offers improved fermentative production of isobutanol from lignocellulosic biomass with a pervaporation procedure.

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Improved production of isobutanol in pervaporation-coupled bioreactor using sugarcane bagasse hydrolysate in engineered Enterobacter aerogenes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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